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Current Genetics

, Volume 26, Issue 5–6, pp 390–397 | Cite as

Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome

  • J. -M. Nicaud
  • A. Raynal
  • A. Beyou
  • M. Merkamm
  • H. Ito
  • N. Labat
Original Articles

Abstract

We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min balf-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttl for an animal-feed additive.

Key words

Overexpression Peroxisomes Saccharomyces cerevisiae Stabilization 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. -M. Nicaud
    • 2
  • A. Raynal
    • 3
  • A. Beyou
    • 1
  • M. Merkamm
    • 1
  • H. Ito
    • 1
  • N. Labat
    • 1
  1. 1.Laboratoire de recherche d'EUROLYSINEParc club Orsay UniversitéOrsayFrance
  2. 2.Laboratoire de Génétique Moléculaire et Cellulaire, Institut National AgronomiqueCBAIThiverval-GrignonFrance
  3. 3.Institut de Génétique et MicrobiologieUniversité d'OrsayOrsay cedexFrance

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