Current Genetics

, Volume 28, Issue 3, pp 258–266 | Cite as

MIG1-dependent and MIG1-independent glucose regulation of MAL gene expression in Saccharomyces cerevisiae

  • Zhen Hu
  • Jan Olof Nehlin
  • Hans Ronne
  • Corinne A. Michels
Original Paper

Abstract

Glucose repression is a global regulatory system in Saccharomyces cerevisiae controlling carbonsource utilization, mitochondrial biogenesis, gluconeogenesis and other metabolic pathways. Mig1p, a zinc-finger class of DNA-binding protein, is a transcriptional repressor regulating GAL and SUC gene expression in response to glucose. This report demonstrates that Mig1 protein represses transcription of the MAL61 and MAL62 structural genes and also the MAL63 gene, which encodes the Malactivator. Mig1p DNA-binding sites were identified upstream of all three MAL genes. Both of the Mig1p-binding sites found in the bidirectional MAL61-MAL62 promoter were shown to function in the Mig1p-dependent glucose repression. Studies using constitutive Mal-activator alleles suggest that glucose regulation of inducer availability is a second major contributing factor in glucose repression of MAL gene expression and is even stronger than the Mig1p-dependent component of repression. Moreover, our results also suggest the contribution of other minor mechanisms in glucose regulation of MAL gene expression.

Key words

Maltose fermentation Glucose repression MIG1 MAL genes 

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References

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994) Current protocols in molecular biology. Greene Publishing Associates, Inc. and John Wiley and Sons, Inc., New YorkGoogle Scholar
  2. Boeke JD, LaCroute F, Fink GR (1984) Mol Gen Genet 197:345–346Google Scholar
  3. Carlson M, Osmond BC, Botstein D (1981) Genetics 98:25–40Google Scholar
  4. Charron MJ, Michels CA (1987) Genetics 116:23–31Google Scholar
  5. Charron MJ, Dubin RA, Michels CA (1986) Mol Cell Biol 6:3891–3899Google Scholar
  6. Dubin RA, Charron MJ, Haut SR, Needleman RB, Michels CA (1989) Mol Cell Biol 8:1027–1035Google Scholar
  7. Estruch F, Carlson M (1993) Mol Cell Biol 13:3872–3881Google Scholar
  8. Flick JS, Johnston M (1990) Mol Cell Biol 10:4757–4769Google Scholar
  9. Flick JS, Johnston M (1992) Genetics 130:295–304Google Scholar
  10. Griggs DW, Johnston M (1991) Proc Natl Acad Sci USA 88:8597–8601Google Scholar
  11. Guarente L (1983) Yeast promoters and LacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol 101:181–191Google Scholar
  12. Johnston M, Carlson M (1992) Regulation of carbon and phosphate utilization. In: Jones EW, Pringle JR, Broach JR (eds) The molecular and cellular biology of the yeast Saccharomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 193–281Google Scholar
  13. Johnston M, Flick JS, Pexton T (1994) Mol Cell Biol 14:3834–3841Google Scholar
  14. Keleher CA, Redd MJ, Schultz J, Carlson M, Johnson AD (1992) Cell 68:709–719Google Scholar
  15. Kim JH, Michels CA (1988) Curr Gener 14:319–323Google Scholar
  16. Lamphier MS, Ptashne M (1992) Proc Natl Acad Sci USA 89:5922–5926Google Scholar
  17. Levine J, Tanouye L, Michels CA (1992) Curr Genet 22:181–189Google Scholar
  18. Lundin M, Nehlin JO, Ronne H (1994) Mol Cell Biol 14:1979–1985Google Scholar
  19. Mathieu M, Felenbok B (1994) EMBO J 13:4022–4027Google Scholar
  20. Myers AM, Tzagaloff A, Kinney DM, Lusty CJ (1986) Gene 45:299–310Google Scholar
  21. Needleman RB (1991) Mol Microbiol 5:2079–2084Google Scholar
  22. Needleman RB, Kaback DB, Dubin RA, Perkins EL, Rosenberg NG, Sutherland KA, Forrest DB, Michels CA (1984) Proc Natl Acad Sci USA 81:2811–2815Google Scholar
  23. Nehlin JO, Ronne H (1990) EMBO J 9:2891–2898Google Scholar
  24. Nehlin JO, Carlberg M, Ronne H (1991) EMBO J 10:3373–3377Google Scholar
  25. Nehlin JO, Carlberg M, Ronne H (1992) Nucleic Acids Res 20:5271–5278Google Scholar
  26. Ni B, Needleman RB (1990) Mol Cell Biol 10:3797–3800Google Scholar
  27. Ronne H (1995) Trends Genet 11:12–17Google Scholar
  28. Tzamarias D, Struhl K (1994) Nature 369:758–760Google Scholar
  29. Trumbly RJ (1992) Mol Microbiol 6:15–21Google Scholar
  30. Vallier LG, Carlson M (1994) Genetics 137:49–54Google Scholar
  31. Yao B, Sollitti P, Zhang X, Marmur J (1994) Mol Gen Genet 243:622–630Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Zhen Hu
    • 1
  • Jan Olof Nehlin
    • 3
  • Hans Ronne
    • 3
  • Corinne A. Michels
    • 1
  1. 1.Department of BiologyQueens College and the Graduate School of CUNYFlushingUSA
  2. 2.Department of BiochemistryFlushingUSA
  3. 3.Ludwig Institute for Cancer Research, Uppsala BranchUppsala Biomedical CenterUppsalaSweden
  4. 4.Lawrence Berkeley LaboratoryDepartment of Cancer BiologyBerkeleyUSA

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