Current Genetics

, Volume 26, Issue 3, pp 228–232

Isolation and characterization of a 1,4-β-endoxylanase gene of A. awamori

  • Johanna G. M. Hessing
  • Co van Rotterdam
  • John M. A. Verbakel
  • Martinus Roza
  • Jan Maat
  • Robert F. M. van Gorcom
  • Cees A. M. J. J. van den Hondel
Original Articles

Abstract

An enzyme with a particular 1,4-β-xylanase activity was identified and purified from wheat-bran culture medium of an Aspergillus awamori strain. With oligonucleotides based on the N-terminal amino-acid sequence of the enzyme, the exlA gene of A. awamori, encoding 1,4-β-xylanase A, has been cloned. Based on the deduced amino-acid sequence, 1,4-β-xylanase A is produced as a 211 amino-acid-residue-long precursor, which is converted post-translationally into a 184-aa-residue-long mature protein. Transformation of the original A. awamori strain with multiple copies of the exlA gene resulted in a 40-fold overproduction of 1,4-β-xylanase A. The overproduced enzyme has the same biochemical and enzymological properties as the wild-type enzyme.

Key words

Aspergillus awamori Enzyme (1,4-β-D-xylanohydrolase E.C. 3.2.1.8) purification exlA gene isolation Multicopy transformant Overexpression 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Johanna G. M. Hessing
    • 1
  • Co van Rotterdam
    • 1
  • John M. A. Verbakel
    • 2
  • Martinus Roza
    • 2
  • Jan Maat
    • 2
  • Robert F. M. van Gorcom
    • 1
  • Cees A. M. J. J. van den Hondel
    • 1
  1. 1.TNO Nutrition and Food ResearchRijswijkThe Netherlands
  2. 2.Unilever Research LaboratoriesVlaardingenThe Netherlands

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