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Surgery Today

, Volume 22, Issue 4, pp 339–345 | Cite as

The proliferative kinetics of somatostatin-producing cells in the rat antral mucosa after truncal vagotomy

  • Hiroshi Shimoda
  • Shigeo Yokoyama
  • Iwao Nakayama
  • Tetsuo Hadama
  • Yuzo Uchida
Original Articles
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Abstract

The kinetics of somatostatin-producing cells (D-cells) in the rat antral mucosa after truncal vagotomy were studied using double immunostaining for bromodeoxyuridine (BrdU) and somatostatin. Both the concentration of somatostatin and D-cell density in the antral mucosa demonstrated a significant increase on the 3rd day after truncal vagotomy. With the single labeling of BrdU, a few D-cells showed positive immunostaining for BrdU throughout the experimental period in both vagotomized and sham operated rats. With cumulative labeling, the BrdU labeled cells demonstrated a linear increase in an identical number for each experimental time-point in both groups. The labeling index of BrdU in the D-cells increased significantly, beginning on the 3rd day and attaining a maximum level of 41.8% on the 10th day, in the vagotomized group after cumulative labeling. In this group, however, the density of D-cells with no immunoreactive BrdU also increased quickly on the 3rd day with cumulative labeling. The present study indicates that the most important factor involving D-cell hyperplasia observed after truncal vagotomy is the activation of pre-existing D-cells to synthesize and release hormones, together with the rapid replication of progenitor cells and their maturation to D-cells.

Key Words

somatostatin-producing cell truncal vagotomy BrdU immunohistochemistry 
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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Hiroshi Shimoda
    • 1
  • Shigeo Yokoyama
    • 2
  • Iwao Nakayama
    • 2
  • Tetsuo Hadama
    • 1
  • Yuzo Uchida
    • 1
  1. 1.The Second Department of SurgeryMedical College of OitaOitaJapan
  2. 2.The First Department of PathologyMedical College of OitaOitaJapan

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