Urological Research

, Volume 21, Issue 4, pp 265–268 | Cite as

Effect of urinary macromolecules on aggregation of calcium oxalate in recurrent calcium stone formers and healthy

  • Shoichi Ebisuno
  • Yasuo Kohjimoto
  • Toshihiko Yoshida
  • Tadashi Ohkawa
Original Articles


The inhibitory activity of urinary macromolecules on the aggregation of calcium oxalate crystals was studied using an aggregometer originally devised to measure thrombocyte aggregation capacity by means of the optical turbidity at 660 nm. The macromolecular fraction of the urine (molecular weight above 5000) of recurrent calcium stone formers showed much less inhibitory activity than that of healthy controls (P0.05). It was speculated on the basis of the results of gel filtration that there were some proteins (molecular weight about 10000–30000) which had inhibitory activities for the aggregation of calcium oxalate. This gives support to the assumption that macromolecules are important during the phase of aggregation of calcium oxalate crystals.

Key words

Aggregometer Calcium oxalate Crystal aggregation inhibitor Normal subjects Stone formers Urinary macromolecules 


  1. 1.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biol 72:248Google Scholar
  2. 2.
    Edyvane KA, Hibberd CM, Harnett RM, Marshall VR, Ryall RL (1987) Macromolecules inhibit calcium oxalate crystal growth and aggregation in whole human urine. Clin Chim Acta 167:329Google Scholar
  3. 3.
    Felix R, Monod A, Broge L, Hansen NM, Fleisch H (1977) Aggregation of calcium oxalate crystals: effect of urine and various inhibitors. Urol Res 5:21Google Scholar
  4. 4.
    Hess B, Nakagawa Y, Coe FL (1989) Inhibition of calcium oxalate monohydrate crystal aggregation by urine proteins. Am J Physiol 257:F99Google Scholar
  5. 5.
    Jelinek ZK (1974) Particle size analysis (Bryce WAJ, translation ed). Wiley, New York, p 70Google Scholar
  6. 6.
    Koide T, Takemoto M, Itatani H, Takaha M, Sonoda T (1981) Urinary macromolecular substances as natural inhibitors of calcium oxalate crystal aggregation. Invest Urol 18:382Google Scholar
  7. 7.
    Robertson WG, Peacock M (1972) Calcium oxalate crystalluria and inhibitors of crystallization in recurrent renal stone formers. Clin Sci 43:499Google Scholar
  8. 8.
    Robertson WG, Peacock M, Nordin BEC (1973) Inhibitors of the growth and aggregation of calcium oxalate in vitro. Clin Chim Acta 43:31Google Scholar
  9. 9.
    Ryall RL, Harnett RM, Marshall VR (1981) The effect of urine, pyrophosphate, citrate, magnesium and glycosaminoglycans on the growth and aggregation of calcium oxalate in vitro. Clin Chim Acta 112:349Google Scholar
  10. 10.
    Ryall RL, Harnett RM, Hibbert CM, Edyvane KA, Marshall VR (1991) Effects of chondroitin sulphate, human serum albumin and Tamm-Horsfall mucoprotein on calcium oxalate crystallization in undiluted human urine. Urol Res 19:181Google Scholar
  11. 11.
    Tiselius HG, Fornander AM, Nilsson MA (1990) Effects of urinary macromolecules on the crystallization of calcium oxalate. Urol Res 18:381Google Scholar
  12. 12.
    Uehara M, Yasukawa S, Ebisuno S, Ohkawa T (1988) New method for determination of urinary oxalate by ion chromatography. Kidney Dial (Jpn) 25:475Google Scholar
  13. 13.
    Yasukawa S, Takamatsu M, Ebisuno S, Morimoto S, Yoshida, Ohkawa T (1985) Studies on citrate metabolism in urolithiasis. I. An enzymatic determination of urinary citrate with citrate lyase. Jpn J Urol 76:1848Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Shoichi Ebisuno
    • 1
  • Yasuo Kohjimoto
    • 1
  • Toshihiko Yoshida
    • 1
  • Tadashi Ohkawa
    • 1
  1. 1.Department of UrologyMinami Wakayama National Hospital and Wakayam Medical CollegeTanabe shiJapan

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