Acta Neuropathologica

, Volume 80, Issue 4, pp 388–392 | Cite as

Incubation periods and histopathological changes in mice injected stereotaxically in different brain areas with the 87V scrapie strain

  • Y. S. Kim
  • R. I. Carp
  • S. Callahan
  • H. M. Wisniewski
Regular Papers


After stereotaxic injection into five different brain areas (cortex, caudate nucleus, substantia nigra, thalamus and cerebellum) of IM mice with the 87V scrapie strain, the cerebellum had the shortes incubation period. The vacuolation pattern was similar regardless of the area injected with extensive vacuolation in the thalamus, mesencephalon and hypothalamus. The pattern of amyloid plaques differed markedly depending on the area injected. In particular, no plaques were seen anywhere in the brain after injection into intact cerebellum, whereas injection into the four cerebral areas yielded plaques in the forebrain but not in the cerebellum. The incubation period after injection into bisected cerebella was much longer than after injection into intact cerebella. Mice injected on one side of bisected cerebellum had amyloid plaques in the forebrain but not in the cerebellum. There is a discussion of the finding that, although no plaques and virtually no vacuolation were seen in the cerebellum, the shortest incubation period occurred after injection into intact cerebellum.

Key words

Scrapie Stereotaxic injection Amyloid plaques Vacuolation Incubation period 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bolton DC, McKinley MP, Prusiner SB (1982) Identification of a protein that purifies with the scrapie prion. Science 218: 1309–1311Google Scholar
  2. 2.
    Bruce ME, Fraser H (1981) Effect of route of injection on the frequency and distribution of cerebral amyloid plaques in scrapie mice. Neuropathol Appl Neurobiol 7: 289–298Google Scholar
  3. 3.
    Bruce ME, Dickinson AG, Fraser H (1976) Cerebral amyloidosis in scrapie in the mouse: effect of agent strain and mouse genotype. Neuropathol Appl Neurobiol 2: 471–478Google Scholar
  4. 4.
    Carp RI, Callahan SM, Sersen EA, Moretz RC (1984) Preclinical changes in weight of scrapie-infected mice as a function of scrapie agent-mouse strain combination. Intervirology 21: 61–69Google Scholar
  5. 5.
    Carp RI, Merz GS, Wisniewski HM (1984) Transmission of unconventional slow virus diseases and the relevance to AD/SDAT transmission studies. In: Wertheimer J, Marois M (es) Senile dementia: outlook for the future. Alan R Liss, New York, pp 31–54Google Scholar
  6. 6.
    Carp RI, Merz PA, Moretz RC, Somerville RA, Callahan SM, Wisniewski HM (1985) Biological properties of scrapie: an unconventional slow virus. In: Maramorosch K, McKelvey JJ (eds) Subviral pathogens of plants and animals: viroids and prions. Academic Press, New York, pp 425–463Google Scholar
  7. 7.
    Carp RI, Moretz RC, Natelli M, Dickinson AG (1987) Genetic control of scrapie: incubation period and plaque formation in I mice. J Gen Virol 68: 401–407Google Scholar
  8. 8.
    Chandler RL (1963) Experimental scrapie in the mouse. Res Vet Sci 4: 276–285Google Scholar
  9. 9.
    Czub M, Braig HR, Diringer H (1986) Pathogenesis of scrapie: study of the temporal development of clinical symptoms, of infectivity titres and scrapie-associated fibrils in brains of hamsters infected intraperitoneally. J Gen Virol 67: 2005–2009Google Scholar
  10. 10.
    Czub M, Braig HR, Diringer H (1988) Relication of scrapie agent in hamsters infected intracerebrally confirms the pathogenesis of an amyloid-inducing virosis. J Gen Virol 69: 1753–1756Google Scholar
  11. 11.
    DeArmond SJ, Mobley WC, DeMott BA, Barry RA, Beckstead JH, Prusiner SB (1987) Changes in the localization of brain prior proteins during scrapic infection. Neurology 37: 1171–1180Google Scholar
  12. 12.
    Dickinson AG (1976) Scrapie in sheep and goats. In: Kimberlin RH (ed) Slow virus diseases of animals and man. North Holland, Amsterdam, pp 209–241Google Scholar
  13. 13.
    Dickinson AG Meikle VMH (1969) A comparison of some biological characteristics of the mouse-passaged scrapie agents, 22A and ME7. Genet Res 13: 213–225Google Scholar
  14. 14.
    Dickinson AG, Fraser H, Bruce ME (1978) Targeting of lesions and variation in agent concentration in different areas of the brain in scrapie. Neuropathol Appl Neurobiol 4: 241Google Scholar
  15. 15.
    Fraser H (1976) The pathology of natural and experimental scrapie. In: Kimberlin RH (ed) Slow virus diseases of animals and man. North Holland, Amsterdam, pp 267–305Google Scholar
  16. 16.
    Fraser H (1979) The neuropathology of scrapie: the precision of the lesions and their diversity. In: Prusiner SB, Hadlow WJ (eds) Slow transmissible diseases of the nervous system, vol 1. Academic Press, New York, pp 387–406Google Scholar
  17. 17.
    Fraser H, Bruce ME (1973) Argyrophilic plaques in mice inoculated with scrapie from particular sources Lancet I: 617–618Google Scholar
  18. 18.
    Fraser H, Dickinson AG (1968) The sequential development of the brain lesions of scrapie in the three strains of mice. J Comp Pathol 78: 301–311Google Scholar
  19. 19.
    Gorde JM, Tamalat J, Toga M, Bert J (1982) Changes in the nigrostriatal system following microinjection of an unconventional agent. Brain Res 240: 87–93Google Scholar
  20. 20.
    Kim YS, Carp RI, Callahan SM, Wisniewski HM (1987) Scrapie-induced obesity in mice. J Infect Dis 156: 402–405Google Scholar
  21. 21.
    Kim YS, Carp RI, Callahan SM, Wisniewski HM (1987) Incubation periods and survival times for mice injected stereotaxically with three scrapic strains in different brain regions. J Gen Virol 68:695–702Google Scholar
  22. 22.
    Kim YS, Carp RI, Callahan SM, Natelli M, Wisniewski HM, (1990) Vaculation, incubation period and survival time analyses in three mouse genotypes injected stereotactically in three brain regions with 22L scrapie strain. J Neuropathol Exp Neurol 49: 106–113Google Scholar
  23. 23.
    Kim YS, Carp RI, Callahan SM, Wisniewski HM (1990) Pathogenesis and pathology of scrapie after stereotactic injection of strain 22L in intact and bisected cerebella. J Neuropathol Exp Neurol 49: 114–121Google Scholar
  24. 24.
    Marsh RF, Sipe JE, Morse SS, Hanson RP (1976) Transmissible mink encephalopathy. Reduced spongiform degeneration in aged mink of the Chediak-Higashi genotype. Lab Invest 34: 381–386Google Scholar
  25. 25.
    Slotnic BM, Leonard CM (1975) A stereotaxic atlas of the albino mouse forebrain. US Department of Health, Education and Welfare, Washington, D.C Publication no. ADM-75-100Google Scholar
  26. 26.
    Wisniewski HM, Bruce ME, Fraser H (1975) Infectious etiology of neuritic (senile) plaques in mice. Science 190: 1108–1110Google Scholar
  27. 27.
    Wisniewski HM, Moretz RC, Lossinski AS (1981) Evidence for induction of localized deposits and neuritic plaques by an infectious agent. Ann Neurol 10: 517–522Google Scholar
  28. 28.
    Wisniewski HM, Vorbrodt AW, Moretz RC, Lossinski AS, Grundke-Iqbal I (1982) Pathogenesis of neuritic (senile) and amyloid plaque formation. Exp Brain Res [Suppl] 5: 3–9Google Scholar
  29. 29.
    Wisniewski HM, Merz GS, Carp RI (1984) Senile dementia of the Alzheimer type: possibility of an infectious etiology in genetically susceptible individuals. Acta Neurol Scand [Suppl] 99: 91–97Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. S. Kim
    • 1
  • R. I. Carp
    • 1
  • S. Callahan
    • 1
  • H. M. Wisniewski
    • 1
  1. 1.New York State Institute for Basic Research in Developmental DisabilitiesStaten IslandUSA

Personalised recommendations