Structure of microtubules: A study of freeze-etched and negatively stained microtubules from the ovaries of Notonecta

  • H. Stebbings
  • J. H. M. Willison
Article

Summary

Insect ovaries of the telotrophic type contain large numbers of microtubules within the tubes which connect an anterior trophic region to each oocyte within the ovariole. We have examined these microtubules using the freeze-etch technique and found that our observations correspond in many ways with the image of microtubules which have been subjected to chemical fixation. Obliquely fractured microtubules show sub-filaments within their walls, while both obliquely and longitudinally fractured microtubules display a periodicity of approximately 4 nm along many of the sub-filaments. In transverse fracture, a “clear zone” can be seen around individual microtubules and this confirms that the “clear zones” which are often seen around transverse sections of microtubules, are real features and not artefacts of fixation.

Key words

Microtubules Notonecta Freeze-etch Substructure 

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References

  1. André, J., Thiéry, J. P.: Mise en évidence d'une sous-structure fibrillaire dans les filaments axonématiques des flagelles. Microscopie 2, 71–80 (1963).Google Scholar
  2. Barnicot, N. A.: A note on the structure of spindle fibres. J. Cell Sci. 1, 217–222 (1966).Google Scholar
  3. Behnke, O., Zelander, T.: Filamentous substructure of microtubules of the marginal bundle of mammalian blood platelets. J. Ultrastruct. Res. 19, 147–165 (1967).Google Scholar
  4. Forer, A.: Chromosome movements during cell division. In: Handbook of molecular cytology (ed. Lima de Faria). Amsterdam: North Holland Publishing Co. 1969.Google Scholar
  5. Gall, J. G.: Microtubule fine structure. J. Cell Biol. 31, 639–643 (1966).Google Scholar
  6. Grimstone, A. V., Cleveland, L. R.: The fine structure of the contractile axostyles of certain flagellates. J. Cell Biol. 24, 387–400 (1965).Google Scholar
  7. Grimstone, A. V., Klug, A.: Observations of the substructure of flagella fibres. J. Cell Sci. 1, 351–362 (1966).Google Scholar
  8. Jensen, C., Bajer, A.: Effects of dehydration on the microtubules of the mitotic spindle. J. Ultrastruct. Res. 26, 367–386 (1969).Google Scholar
  9. Kane, R. E.: The mitotic apparatus. Fine structure of the isolated unit. J. Cell Biol. 15, 279–287 (1962).Google Scholar
  10. Kiefer, B., Sakai, H., Solari, A. J., Mazia, D.: The molecular unit of the microtubules of the mitotic apparatus. J. molec. Biol. 20, 75–79 (1966).Google Scholar
  11. Kirkpatrick, J. B.: Microtubules in brain homogenates. Science 163, 187–188 (1969).Google Scholar
  12. Lane, N. J., Treherne, J. E.: Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci. 7, 217–231 (1970).Google Scholar
  13. Ledbetter, M. C., Porter, K. R.: A “microtubule” in plant cell fine structure. J. Cell Biol. 19, 239–250 (1963).Google Scholar
  14. Ledbetter, M. C., Porter, K. R.: Morphology of microtubules of plant cells. Science 144, 872–874 (1964).Google Scholar
  15. Macgregor, H. C., Stebbings, H.: A massive system of microtubules associated with cytoplasmic movement in teletrophic ovarioles. J. Cell Sci. 6, 431–449 (1970).Google Scholar
  16. Markham, R., Frey, S., Hills, G.: Methods for the enhancement of image detail and accentuation of structure in electron microscopy. Virology 20, 88–102 (1963).Google Scholar
  17. Maser, M. D., Philpott, C. W.: Marginal bands in nucleated erythrocytes. Anat. Rec. 150, 365–381 (1964).Google Scholar
  18. Moor, H.: Der Feinbau der Mikrotubuli in Hefe nach Gefrierätzung. Protoplasma (Wien) 64, 89–103 (1967).Google Scholar
  19. Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963).Google Scholar
  20. Northcote, D. H., Lewis, D. R.: Freeze-etched surfaces of membranes and organelles in the cells of pea root tips. J. Cell Sci. 3, 199–206 (1968).Google Scholar
  21. Pickett-Heaps, J. D., Northcote, D. H.: Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J. Cell Sci. 1, 109–120 (1966).Google Scholar
  22. Porter, K. R.: Cytoplasmic microtubules and their function. In: Principles of bimolecular organisation, eds. G. E. W. Wolstenholme and M. O'Connor, p. 308–356. London: J. and A. Churchill 1966.Google Scholar
  23. Roth, L. E., Pihlaja, D. J., Shigenaka: Microtubules in the heliozoan axopodium. I. The gradion hypothesis of allasterism in structural proteins. J. Ultrastruct. Res. 30, 7–37 (1970).Google Scholar
  24. Silver, M. D., McKinstry, J. E.: Morphology of microtubules in rabbit platelets. Z. Zellforsch. 81, 12–17 (1967).Google Scholar
  25. Smith, D. S.: On the significance of cross-bridges between microtubules and synaptic vesicles. Phil. Trans. B 261, 395–405 (1971).Google Scholar
  26. Tilney, L. G., Byers, B.: Studies on the microtubules in heliozoa. J. Cell Biol. 43, 148–165 (1969).Google Scholar
  27. Tucker, J. B.: Fine structure and function of the cytopharyngeal basket of the ciliate Nassula. J. Cell Sci. 3, 493–514 (1968).Google Scholar
  28. Tucker, J. B.: Microtubule-arms and propulsion of food particles inside a large feeding organelle in the ciliate Phascolodon vorticella. J. Cell Sci. 10, 883–903 (1972).Google Scholar
  29. Willison, J. H. M., Cocking, E. C.: Frozen fractured viruses; a study of virus structure using freeze etching. J. Microsc. 95, 397–411 (1972).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. Stebbings
    • 1
    • 2
    • 3
  • J. H. M. Willison
    • 1
    • 2
  1. 1.Department of Zoology, Adrian BuildingUniversity of LeicesterLeicester
  2. 2.Department of BotanyUniversity of NottinghamNottinghamEngland
  3. 3.Department of BiologyUniversity of ExeterExeterEngland

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