Summary
The histochemioal distribution of sorbitol dehydrogenase in normal and cryptorchid rat testis has been studied. In the normal testis sorbitol dehydrogenase is localized in the spermatids, increases during their differentiation and is maximal in those spermatids attached to the Sertoli cells (stages V–VII). In the cryptorchid testis, sorbitol dehydrogenase activity of the spermatids, similarly to that of the Sertoli cells, is completely abolished. Therefore, we conclude that sorbitol dehydrogenase activity of the Sertoli cells depends on the spermatid differentiation.
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Abbreviations
- SBDH:
-
sorbitol dehydrogenase
- NAD:
-
nicotinamide adenine dinucleotide
- NBT:
-
m-nitroneotetrazolium chloride
- PMS:
-
phenazine methosulfate
- Tris:
-
tris (hydroxymethyl) aminomethane
References
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Germino, N.I., Ohanian, C., Gastel, M.T. et al. Effect of experimental cryptorchism on testis L-iditol: NAD oxidoreductase (sorbitol dehydrogenase or ketose-reductase). Histochemie 37, 19–22 (1973). https://doi.org/10.1007/BF00306856
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DOI: https://doi.org/10.1007/BF00306856