Ultrastructural localization of glycogen in erythrocytes and developing erythrocytic cells in normal human bone marrow

  • G. Adolph Ackerman
Article
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Summary

The periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) reaction was employed for the ultrastructural cytochemical localization of saliva-labile glycogen in the erythrocytic cells in normal human blood and bone marrow. Particulate glycogen was demonstrated in the cytoplasm of all developmental forms of erythrocytic cells from the proerythroblast through the reticulocyte; a few particles of glycogen also were present in mature erythrocytes even in the peripheral blood. Statistical evaluation of the number of glycogen particles in mid-plane cell sections at each morphological stage of development indicated a significant and stepwise decrease during cellular maturation. This change in glycogen content may reflect both cellular utilization and mitosis during the maturational sequence.

Key words

Bone marrow Erythrocyte Erythropoiesis Glycogen Ultrastructural cytochemistry 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • G. Adolph Ackerman
    • 1
  1. 1.Department of AnatomyThe Ohio State UniversityColumbusUSA

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