Protein staining of ribboned epon sections for light microscopy
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Procedures are described for obtaining and handling ribboned epon sections 0.3–2 μ thick for light microscopy, and for the cytological application of two intense acid dyes, Aniline Blue Black and Coomassie Brilliant Blue R 250. The technique allows precise localization of proteins and some other materials, and, because the sections are ribboned, facilitates three-dimensional visualization of the structures involved. The dyes may be used in combination with the periodic acid-Schiff reaction and with autoradiography.
KeywordsPublic Health Light Microscopy Aniline Coomassie Brilliant Blue
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