, Volume 16, Issue 1, pp 92–96 | Cite as

Protein staining of ribboned epon sections for light microscopy

  • Donald B. Fisher


Procedures are described for obtaining and handling ribboned epon sections 0.3–2 μ thick for light microscopy, and for the cytological application of two intense acid dyes, Aniline Blue Black and Coomassie Brilliant Blue R 250. The technique allows precise localization of proteins and some other materials, and, because the sections are ribboned, facilitates three-dimensional visualization of the structures involved. The dyes may be used in combination with the periodic acid-Schiff reaction and with autoradiography.


Public Health Light Microscopy Aniline Coomassie Brilliant Blue 


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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Donald B. Fisher
    • 1
  1. 1.Department of BotanyUniversity of CaliforniaBerkeley

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