Theoretical and Applied Genetics

, Volume 77, Issue 3, pp 325–331

DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs

  • F. LoSchiavo
  • L. Pitto
  • G. Giuliano
  • G. Torti
  • V. Nuti-Ronchi
  • D. Marazziti
  • R. Vergara
  • S. Orselli
  • M. Terzi
Article

Summary

The level of auxin - both natural and synthetic — in the medium has a strong effect on the level of 5-methyl-cytosine in the DNA of carrot cells in culture. This level may vary from approximately 15% to 70% of total cytosine without apparent effects on growth rate and cell morphology. No effect was seen with cytokinin. During somatic embryogenesis, in the absence of hormones, variations were seen in the level of methylation according to a characteristic pattern. If hypomethylation is induced with drugs such as azacytidine, ethionine or ethoxy-carbonyl-pyrimidine, embryogenesis is immediately blocked. A mutant was isolated which is resistant to the action of hypomethylating drugs. It shows variations in the methylation pattern and variations in indole-acetic acid metabolism. In addition its regeneration is often associated with the production of tumors.

Key words

Methylation Somatic embryogenesis Auxin Tumors Developmental mutants 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • F. LoSchiavo
    • 1
  • L. Pitto
    • 2
  • G. Giuliano
    • 2
  • G. Torti
  • V. Nuti-Ronchi
    • 2
  • D. Marazziti
    • 2
  • R. Vergara
    • 2
  • S. Orselli
    • 2
  • M. Terzi
    • 3
  1. 1.Istituto Internazionale di Genetica e Biofisica, CNRNapoliItaly
  2. 2.Istituto di Mutagenesi e Differenziamento, CNRPisaItaly
  3. 3.Dipartimento di Genetica, Biologia Generale e MolecolareUniversità di NapoliItaly

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