The effects of some carcinogenic nitroso compounds on the rat liver nucleolus

  • Kiyoshi Terao


The hepatocyte nucleoli of rats undergo a variety of specific and nonspecific alterations after the administration of 8 nitroso compounds and 5 corresponding non-nitroso compounds. After the oral administration of dimethylnitrosamine and diethylnitrosamine, an unusual segregation of the nucleolus was encountered in the cells with karyorrhexis. Most characteristic of the segregation are the scattered ribosome-like granules at the outermost layer of the altered nucleolus. Frequently microspherules with halos 150 nm in diameter are found in all rats treated with nitroso compounds, which have been reported to be carcinogenic in the organs other than the liver. With the exception of butylurea and butylamine no such specific microspherules were found in the nucleoli after the administration of coresponding non-nitroso compounds.


Public Health Cancer Research Oral Administration Nitroso Outermost Layer 
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Über die Wirkung einiger cancerogener Nitroso-Verbindungen auf die Nukleolen von Leberzellen der Ratte


Die Nucleolen von Leberzellen der Ratte erleiden nach Gaben von 8 verschiedenen Nitrosoverbindungen und 5 entsprechenden Nicht-Nitrosoverbindungen eine Vielfalt von spezifischen und unspezifischen Veränderungen. Nach oraler Zufuhr von Dimethylnitrosamin und Diäthylnitrosamin wurde eine ungewöhnliche Segregation der Nukleolen in den Zellen mit Karyorrhexis beobachtet. Sehr kennzeichnend für diese Segregation sind verstreute Ribosomen-artige Granula in der äußersten Schicht der veränderten Nucleolen. Oft sind Mikrosphären mit Halos 150 nm in allen Ratten zu finden, die mit Nitrosoverbindungen behandelt wurden, welche als carcinogen für andere Organe als die Leber beschrieben wurden. Mit Ausnahme von Butylharnstoff und Butylamin wurden keine solche spezifischen Mikrosphären in den Nucleoli nach der Verabreichung der korrespondierenden Nicht-Nitrosoverbindungen beobachtet.


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  1. Bouteille,M., Laval,M., Dupuy-Coin,A.M.: Localization of nuclear functions as revealed by ultrastructural autoradiography and cytochemistry. In: Busch,H. (Ed.): The Cell Nucleus, Vol. 1, p. 1. New York-London: Academic Press 1974Google Scholar
  2. Busch,H., Smetana,K.: The Nucleolus. New York-London: Academic Press 1970Google Scholar
  3. Daskal,Y., Crooke,S.T., Smetana,K., Busch,H.: Ultrastructural study of the effect of Bleomycin A2 on the nucleolus and its possibly related cytoplasmic constituents in Novikoff hepatoma cells. Cancer Res. 35, 374–381 (1975)Google Scholar
  4. Fium,L.: Pathogenesis of the cellular lesions produced by α-amanitin. In: Farber,E. (Ed.): The Pathology of Transcription and Translation, pp. 105–122. New York: Marcel Dekker Inc. 1972Google Scholar
  5. Goldblatt,P.J., Sullivan,R.J.: Sequential morphological alterations in hepatic cell nucleoli induced by varying doses of actinomycin D. Cancer Res. 30, 1349–1356 (1970)Google Scholar
  6. Lombardi,L.: Ultrastructural study of nucleolar alterations produced by a single carcinogenic dose of urethan in mouse hepatocytes. Cancer Res. 32, 675–679 (1972)Google Scholar
  7. Magee,P.N., Farber,E.: Toxic liver injury and carcinogenesis; methylation of rat-liver nucleic acids by dimethylnitrosoamine in vivo. Biochem. J. 83, 114–124 (1962)Google Scholar
  8. Recher,L., Chan,H., Briggs,L., Parry,N.: Ultrastructural changes inducible with the plant alkaloid camptothecin. Cancer Res. 32, 2495–2501 (1972)Google Scholar
  9. Reynolds,R.C., Montgomerry,P.O'B., Karney,D.: Nucleolar “cap” — a morphological entity produced by the carcinogen 4-nitroquinoline N-oxide. Cancer Res. 23, 535–538 (1963)Google Scholar
  10. Shinozuka,H.: Response of nucleus and nucleolus to inhibition of RNA synthesis. In: Farber,E. (Ed.): The Pathology of Transcription and Translation, pp.73–103. New York: Marcel Dekker Inc. 1972Google Scholar
  11. Svoboda,D., Higginson,J.: A comparison of ultrastructural changes in rat liver due to chemical carcinogens. Cancer Res. 28, 1703–1733 (1968)Google Scholar
  12. Terao,K.: Massive and single cell necrosis in the rat liver induced by aflatoxin B1 and sterigmatocystin. Acta Path. Jap. 23, 647–653 (1973)Google Scholar
  13. Terao,K.: Unpublished DataGoogle Scholar
  14. Villa-Trevino,S.: Comparison of some biochemical alterations produced by hepatotoxic agents. Biochem. J. 96, 62–63 (1965)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Kiyoshi Terao
    • 1
  1. 1.Research Institute for ChemobiodynamicsChiba UniversityChibaJapan

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