Theoretical and Applied Genetics

, Volume 70, Issue 3, pp 248–251 | Cite as

Regeneration of peach plants from callus derived from immature embryos

  • F. A. Hammerschlag
  • G. Bauchan
  • R. Scorza
Article

Summary

Peach plants were repeatedly regenerated from immature embryos but not from callus derived from mature embryos. A white, nodular, highly regenerative callus was obtained when friable, primary callus from immature embryos was transferred from medium containing 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.44 μM benzyladenine (BA) to media containing 0.27 μM α-naphthaleneacetic acid (NAA) and 2.2 μM BA. This callus retained its morphogenetic potential for a minimum of three subcultures. Green nodular callus, that lacked regenerative capacity, was produced from primary callus derived from mature embryos. Maximum regeneration of shoots occurred when highly regenerative callus was transferred to a medium in which the NAA concentration was reduced five times and the BA concentration was increased two times. Regenerated shoots were rooted in the dark on a medium containing 28.5 μM indoleacetic acid. Cytogenetic analysis of regenerated plants indicated that all plants were diploid, 2n = 2x = 16. Phenotypic evaluation of regenerated plants, grown under field conditions, is now in progress.

Key words

Prunus persica Peach Embryo culture Plant regeneration Cytogenetic analysis 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. A. Hammerschlag
    • 1
  • G. Bauchan
    • 1
  • R. Scorza
    • 2
  1. 1.Tissue Culture and Molecular Genetics Laboratory and Field Crops LaboratoryBARC-West, USDA, ARSBeltsvilleUSA
  2. 2.Appalachian Fruit Research Station, USDA, ARSKearneysvilleUSA

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