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European Journal of Nuclear Medicine

, Volume 4, Issue 4, pp 271–275 | Cite as

The mechanism of the localization of 67Gallium citrate in experimental abscesses

  • Peter A. G. Hammersley
  • David M. Taylor
Article

Abstract

67Ga accumulation in turpentine-induced abscesses, from 5 h to 20 days old, in the rat has been investigated, and the results indicate that this is dependent on polymorphonuclear leukocyte (PMN) infiltration of the inflamed site. 67Ga concentration 24 h after injection correlates well with the activities of the lysosomal enzymes, aryl sulphatase and β-glucuronidase, P≪0.001, and with the DNA content of the tissue. There is no direct relationship with the rate of DNA synthesis.

The mechanism of lysosomal incorporation into PMNs is consistent with the factors involved in 67Ga uptake into tumours and normal tissues.

Keywords

Polymorphonuclear Leukocyte Turpentine Lysosomal Enzyme Activity 67Ga Uptake 67Ga Accumulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aulbert, E., Haubold, U.: Isolation of the 67Gallium accumulating fraction in normal rat liver. Nucl. Med. (Stuttg.) 13, 72–84 (1974)Google Scholar
  2. Burleson, R.L., Johnson, M.C., Head, H.: In vitro and in vivo labelling of rabbit blood leukocytes with 67Ga citrate. J. Nucl. Med. 15, 98–101 (1974)PubMedGoogle Scholar
  3. Dhawan, V.M., Sziklas, J.J., Spencer, R.P.: Localisation of Ga-67 in inflammations in the abscence of polymorphonuclear leukocytes. J. Nucl. Med. 19, 292–294 (1978)PubMedGoogle Scholar
  4. Edwards, C.L., Hayes, R.L.: Tumour scanning with 67Ga citrate. J. Nucl. Med. 10, 103–105 (1969)PubMedGoogle Scholar
  5. Gelrud, L.G., Arseneau, J.C., Milder, M.S., Kramer, R.J., Swann, S.J., Canellos, G.P., Johnston, G.S.: The kinetics of 67Gallium incorporation into inflammatory lesions: experimental and clinical studies. J. Lab. Clin. Med. 83, 489–495 (1974)PubMedGoogle Scholar
  6. Hammersley, P.A.G., Cauchi, M.N., Taylor, D.M.: Uptake of 67Ga in the regenerating rat liver and its relationship to lysosomal enzyme activity. Cancer Res. 35, 1154–1158 (1975)PubMedGoogle Scholar
  7. Hammersley, P.A.G., Taylor, D.M.: The role of lysosomal enzyme activity in the localisation of 67Gallium citrate. Eur. J. Nucl. Med 4, 261–270 (1979)CrossRefPubMedGoogle Scholar
  8. Harvey, W.C., Podoloff, D.A., Kopp, D.T.: 67Ga in 68 consecutive infection searches. J. Nucl. Med. 16, 2–4 (1975)PubMedGoogle Scholar
  9. Haubold, U., Aulbert, E.: Gallium-67 as a tumour scanning agent. In: Medical Radioisotope Scintigraphy p. 553–564. Vienna: IAEA 1972Google Scholar
  10. Higasi, T., Nakayama, Y., Muraka, A., Nakamura, K., Sugiyama, M., Kawaguchi, T., Suzuki, S.: Clinical evaluation of 67Ga citrate scanning. J. Nucl. Med. 13, 196–201 (1972)PubMedGoogle Scholar
  11. Hopkins, G.B., Kan, M., Mende, C.W.: Early 67Ga scintigraphy for the localisation of abdominal abscesses. J. Nucl. Med. 16, 990–992 (1975)PubMedGoogle Scholar
  12. Lavender, J.P., Lowe, J., Barker, J.R., Burn, J.I., Chaudhri, M.A.: Gallium-67 scanning in neoplastic and inflammatory lesions. Br. J. Radiol. 44, 361–366 (1971)CrossRefPubMedGoogle Scholar
  13. Paterson, A.H.G., Yoxall, A., Smith, I., Taylor, D.M., McCready, V.R.: The concentration of 67Ga and 45Ca in the lactating mammary gland and its relevance to the tumour uptake of 67Ga citrate. Cancer Res. 36, 452–457 (1976)PubMedGoogle Scholar
  14. Swartzendruber, D.C., Nelson, B., Hayes, R.L.: Gallium-67 localisation in lysosome-like granules of leukaemic and non-leukaemic murine tissue. J. Natl. Cancer Inst. 46, 941–952 (1971)PubMedGoogle Scholar
  15. Takeda, S., Uchida, T., Matzuzawa, T.: A comparative study of lysosomal accumulation of gallium-67 and indium-111 in Morris hepatoma 7316A. J. Nucl. Med. 18, 835–839 (1977)PubMedGoogle Scholar
  16. Tsan, M.-F., Chen, W.Y., Scheffel, UI, Wagner, Jr., H.N.: Studies on gallium accumulation in inflammatory lesions: I. Gallium uptake by human polymorphonuclear leukocytes. J. Nucl. Med. 19, 36–43 (1978)PubMedGoogle Scholar
  17. Tsan, M.-F.: Studies on gallium accumulation in inflammatory lesions: III. Roles of polymorphonuclear leukocytes and bacteria. J. Nucl. Med. 19, 492–495 (1978)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Peter A. G. Hammersley
    • 1
  • David M. Taylor
    • 1
  1. 1.Radiopharmacology Department, Institute of Cancer ResearchRoyal Marsden HospitalSuttonU.K.

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