Cell and Tissue Research

, Volume 273, Issue 1, pp 71–77 | Cite as

Insulin-like molecules in the beetle Tenebrio molitor

  • V. M. Sevala
  • V. L. Sevala
  • B. G. Loughton


Immunocytochemical staining of the nervous system of larva, pupa, and adult stage of Tenebrio molitor with anti-insulin serum demonstrated insulin-like peptides in the protocerebrum, corpora allata, and suboesophageal ganglion. During pupal development, marked changes in staining intensity of the protocerebral cells were detected. The staining pattern suggests release of insulin-like peptides early on day 0 and again on day 3 of the stadium. Injections of anti-insulin at these times caused significant delays in the timing of pupal/adult ecdysis. An immunoblot of haemolymph from day-3 pupae revealed a 6.5-kDa insulin-like molecule. These results suggest that the prothoracicotropic hormone of T. molitor is an insulin-like molecule.

Key words

Insulin-like peptides Pupa Moulting Tenebrio molitor (Insecta) 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • V. M. Sevala
    • 1
  • V. L. Sevala
    • 1
  • B. G. Loughton
    • 1
  1. 1.Department of BiologyYork UniversityNorth YorkCanada

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