Advertisement

Histochemie

, Volume 10, Issue 4, pp 329–335 | Cite as

Zur Histochemie von Hydrolasen im Hoden des Hundes und der Katze

  • Karl -Heinz Wrobel
  • Wolfgang Kühnel
Article

Zusammenfassung

Wir untersuchten das Verteilungsmuster von unspezifischer Esterase, alkalischer Phosphatase, Adenosintriphosphatase, 5′-Nucleotidase und β-D-Glucuronidase im Hoden von Hund und Katze. Besonders hervorzuheben sind eine starke Aktivität der unspezifischen Esterase in den Sertolizellen der Katze, der Reichtum der Membrana propria aller Hodentubuli an alkalischer Phosphatase und Adenosintriphosphatase sowie die kräftige Reaktion auf β-D-Glucuronidase in den Leydigzellen beider Tierarten.

Die Befunde werden diskutiert.

Summary

The localization of unspecific esterase, alkaline phosphatase, adenosine triphosphatase, 5′-nucleotidase, and β-D-glucuronidase in the testes of cat and dog was demonstrated by histochemical means. We observed a strong esterase activity in the Sertoli cells of the cat and high amounts of alkaline phosphatase and adenosine triphosphatase in the membrana propria of all seminiferous tubules. In both species the principal site of β-D-glucuronidase was in the Leydig cells. Our findings obtained being discussed.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Baust, P., H. G. Goslar u. E. Tonutti: Das Verhalten der Esterasen im Hoden von Ratte, Maus und Meerschweinchen nach Östrogenbehandlung. Z. Zellforsch. 69, 686–698 (1966).Google Scholar
  2. Billet, F., and S. M. McGee-Russel: The histochemical localization of β-glucuronidase in the liver of the newt. Quart. J. micr. Sci. 97, 155–159 (1956).Google Scholar
  3. Braun-Falco, O.: Zur Histotopographie der β-Glucuronidase in normaler menschlicher Haut. Arch. klin. exp. Derm. 203, 61–67 (1956).Google Scholar
  4. Campbell, J. G.: The intracellular localization of β-glucuronidase. Brit. J. exp. Path. 30, 548–554 (1949).Google Scholar
  5. Chauncey, H. H., and G. Quintarelli: Localization of acid phosphatase, nonspecific esterase and β-D-galactosidase in parotid and submaxillary glands of domestic and laboratory animals. Amer. J. Anat. 108, 263–293 (1961).Google Scholar
  6. Deimling, O. H. v.: Die Darstellung phosphatfreisetzender Enzyme mittels Schwermetall-Simultan-Methode. Histochemie 4, 48–55 (1964).Google Scholar
  7. Fishman, W. H.: β-Glucuronidase. In: Advances in enzymology (ed. by F. F. Nord), vol. 26. New York and London: Interscience Publ. 1955.Google Scholar
  8. —: Rat skeleton muscle β-glucuronidase localization by postcoupling technique using naphthol AS-BI, β-D-glucosiduronic acid and fast blue R. J. Histochem. Cytochem. 4, 306–308 (1956).Google Scholar
  9. —, and A. J. Anlyan: β-glucuronidase activity in human tissues. Cancer Res. 7, 808–817 (1947).Google Scholar
  10. —, and J. R. Baker: Cellular localization of β-glucuronidase in rat tissues. J. Histochem. Cytochem. 4, 570–587 (1956).Google Scholar
  11. —, and L. W. Fishman: The elevation of uterine β-glucuronidase activity by estrogenic hormones. J. biol. Chem. 152, 487–488 (1944).Google Scholar
  12. —, St. S. Goldman, and S. Green: Several biochemical criteria for evaluating β-glucuronidase localization. J. Histochem. Cytochem. 4, 239–251 (1956).Google Scholar
  13. Gomori, G.: Distribution of lipase in the tissues under normal and pathologic conditions. Arch. Path. 41, 121–129 (1946).Google Scholar
  14. —: Histochemical specifity of phosphatases, Proc. Soc. exp. Biol. (N.Y.) 70, 7–11 (1949).Google Scholar
  15. Hayashi, M., J. Nakajima, and W. H. Fishman: The cytologie demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report. J. Histochem. Cytochem. 4, 293–297 (1956).Google Scholar
  16. —, K. Ogata, T. Shiraogawa, Z. Murakami, and S. Tuneishi: The effect of unilateral and bilateral cryptorchidisms upon the activities of β-glucuronidase, lipase and esterase in the rat testis. Acta path. jap. 8, 113–126 (1958).Google Scholar
  17. —, K. Simoda, K. Ogata, T. Takamori, T. Shiraogawa, and O. Kawase: Histochemical studies on the uterine β-glucuronidase, sulfatage and esterase activities during the rat estrous cycle. Acta histochem. (Jena) 3, 277–283 (1956).Google Scholar
  18. Hosoyama, Y., and T. Shimadate: Alkaline phosphatase of rat testis. Zool. Mag. 75, 71–76 (1966).Google Scholar
  19. Huggins, Ch., and St. H. Moulton: Esterases of testis and other tissues. J. exp. Med. 88, 169–179 (1948).Google Scholar
  20. Kagawa, S., and K. Osanai: Histochemical demonstration of hydrolytic enzymes and dehydrogenases in the oral mucosa of cats. J. Histochem. Cytochem. 11, 635–640 (1963).Google Scholar
  21. Kawakatsu, K., M. Mori, K. Fujita, M. Fukuda, and S. Deguchi: Histochemical studies on salivary glands. VI. Histochemical demonstration of β-glucuronidase, β-galactosidase and β-glucosidase in normal salivary gland. Arch. Histol. jap. 19, 533–546 (1960).Google Scholar
  22. —, M. Fukuda, S. Kagawa, and K. Osanai: Histochemical studies of the developing submaxillary gland in the human fetus. J. Osaka Univ. dent. Soc. 3, 3–9 (1963).Google Scholar
  23. Kühnel, W., u. K.-H. Wrobel: über die histochemisch faßbare Aktivität der β-D-Glucuronidase und der β-D-Galaktosidase in der Harderschen Drüse des Kaninchens. Albrecht v. Graefes Arch. Ophthal. 171, 173–183 (1966).Google Scholar
  24. Leonhard, S. L., and E. Knobil: β-Glucuronidase activity in the rat uterus. Endocrinology 47, 331–337 (1950).Google Scholar
  25. Mancini, R. E., J. Nolazco, and F. A. de la Balze: Histochemical study of normal adult human testis. Anat. Rec. 114, 127–147 (1952).Google Scholar
  26. Montagna, W.: Histology and cytochemistry of human skin. XI. The distribution of β-glucuronidase. J. biophys. biochem. Cytol. 3, 343–348 (1957).Google Scholar
  27. Nachlas, M. M., D. T. Crawford, and A. M. Seligman: The histochemical demonstration of leucine aminopeptidase. J. Histochem. Cytochem. 5, 264–278 (1957).Google Scholar
  28. —, and A. M. Seligman: The comparative distribution of esterase in the tissues of five mammals by a histochemical technique. Anat. Rec. 105, 677–696 (1949).Google Scholar
  29. Niemi, M., M. Härkönen, and A. Kokko: Localization and identification of testicular esterases in the rat. J. Histochem. Cytochem. 10, 186–193 (1962).Google Scholar
  30. Odell, O. D., and W. H. Fishman: Studies on β-Glucuronidase. I. Activities in human endometrium. Amer. J. Obstet. Gynec. 59, 200–203 (1950).Google Scholar
  31. Ogata, T., and M. Mori: A histochemical study of hydrolytic enzymes in muscle fibers of various animals. J. Histochem. Cytochem. 11, 645–652 (1963).Google Scholar
  32. Schmidt, F. C.: Licht-und elektronenmikroskopische Untersuchungen am menschlichen Hoden und Nebenhoden. Z. Zellforsch. 63, 707–727 (1964).Google Scholar
  33. Seligman, A. M., K.-Ch. Tsou, S. H. Rutenburg, and R. B. Cohen: Histochemical demonstration of β-Glucuronidase with a synthetic substrate. J. Histochem. Cytochem. 2, 209–229 (1954).Google Scholar
  34. Shantaveerappa, T. R., and G. H. Bourne: Histochemical studies on the distribution of β-glucuronidase in the eye. Histochemie 3, 413–421 (1964).Google Scholar
  35. Steigleder, G. K.: Über den Nachweis der β-Glucuronidase auf der Hautoberfläche. Klin. Wschr. 36, 984–985 (1958).Google Scholar
  36. Verne, J., et S. Hébert: L'activité esterasique des cellules interstitielles du testicule et de l'épididyme chez le rat. Ann. Endocr. (Paris) 13, 924–930 (1952).Google Scholar
  37. Wrobel, K.-H., u. W. Kühnel: Die Histotopik einiger Oxydoreductasen im Hoden von Hund und Katze. Histochemie 10, 208–215 (1967).Google Scholar

Copyright information

© Springer-Verlag 1967

Authors and Affiliations

  • Karl -Heinz Wrobel
    • 1
  • Wolfgang Kühnel
    • 1
  1. 1.Anatomisches Institut der Universität Marburg a.d. LahnDeutschland

Personalised recommendations