Urological Research

, Volume 24, Issue 3, pp 171–175 | Cite as

Functional restoration of rat bladder after subtotal cystectomy: in vivo cystometry and in vitro study of whole bladder

  • M. Saito
  • M. Ohmura
  • K. Yokoi
  • A. Kondo
  • Y. Yoshikawa
Original Paper


Functional restoration of the rat urinary bladder following subtotal cystectomy was studied via in vivo infusion cystometry and an in vitro whole bladder model. After the bladder had been separated from the prostate, subtotal cystectomy was achieved by ligating the bladder completely at a level just above the insertion of the ureters into the bladder. Bladder function was investigated immediately and 7, 14, and 28 days after surgery. Bladder weight was reduced to 17% that in sham-operated controls immediately after surgery, but recovered to 76% of that in controls 28 days after the operation. In vivo capacity also increased after surgery from 13% that of controls to 59% 28 days later. However, voiding pressure remained low (34% of control) 28 days later. An in vitro whole bladder study showed that the response to field stimulation decreased significantly on day 7, but had recovered considerably by day 28. The maximal response to bethanechol decreased significantly 7 days after surgery, but recovered thereafter. The response to phenylephrine increased significantly immediately after surgery, but gradually returnd to the control level. An in vitro volume-pressure study showed that passive complance of the cystectomized bladder decreased after surgery, but improved with time. The peak of the active pressure increase to field stimulation occurred at a low infusion volume immediately after surgery, but bladder capacity increased gradually until 28 days later, when the maximal active pressure was obtained. Our results suggest that restoration of the bladder following subtotal cystectomy may not derive simply from an expansion of the bladder wall. Functional alteration involving the bladder base was also observed.

Key words

Rat bladder Subtotal cystectomy Bladder regeneration Whole bladder Infusion cystometry 


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  1. 1.
    Alm P, Elmer M (1975) Adrenergic and cholinergic innervation of the rat urinary bladder. Acta Physiol Scand 94:36Google Scholar
  2. 2.
    Baker R, Kelly T, Tehan T, Putnam C, Beaugard C, Beaugard E (1958) Subtotal cystectomy and total bladder regeneration in treatment of bladder cancer. JAMA 168:1178Google Scholar
  3. 3.
    El-Badawi A, Schenk EA (1966) Dual innervation of the mammalian urinary bladder. Am J Anat 119:405Google Scholar
  4. 4.
    Gasser TC, Larsen EH, England DM, Graversen PH, Dorflinger T, Madsen PO, Brukewitz RC (1987) Urinary bladder reformation: regeneration or dilatation? Neurourol Urodyn 6:129Google Scholar
  5. 5.
    Goldstein AMB, Gualtieri V, Getzoff PL (1970) Expansion mechanisms of the partially cystectomized bladder: an experimental study in rabbits. J Urol 104:413Google Scholar
  6. 6.
    Kretchmer HL, Barber KE (1928) Regeneration of the bladder following resection. JAMA 90:355Google Scholar
  7. 7.
    Liang DS, Goss RJ (1963) Regeneration of the bladder after subtotal cystectomy in rats. J Urol 89:427Google Scholar
  8. 8.
    Lin ATL, Kato K, Monson FC, Wein AJ, Levin RM (1989) Pharmacological responses of rabbit urinary bladder after subtotal cystectomy. J Urol 142:409Google Scholar
  9. 9.
    Ross G, Thompson IM, Bynum WR, Thompson EP (1966) The role of smooth muscle regeneration in urinary tract repair. J Urol 95:541Google Scholar
  10. 10.
    Schiller H (1923) Regeneration of resected urinary bladders in rabbits. Surg Gynec Obst 36:24Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Saito
    • 1
  • M. Ohmura
    • 1
  • K. Yokoi
    • 1
  • A. Kondo
    • 1
  • Y. Yoshikawa
    • 2
  1. 1.Department of UrologyNagoya University School of MedicineNagoyaJapan
  2. 2.Department of UrologyHekinan Municipal HospitalHekinanJapan

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