Untersuchungen zum cytostatischen Wirkungsmechanismus der Vitamin-A-Säure

  • A. Jung
  • E. Bauer
  • K. Lenger
  • A. Scholz
  • R. Jackisch
Article
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Zusammenfassung

Die cytostatischen Effekte der Vitamin-A-Säure auf Ehrlich-Ascites-Tumorzellen in-vitro, sowie auf einige zellfreie Enzymsysteme wurden untersucht. Vitamin-A-Säure beeinflußt konzentrationsabhängig, jedoch unspezifisch die Einbauraten von 14C-Thymidin,-Uridin und -Leucin. Teilweise gereinigte Thymidinkinase aus Ascites-Tumorzellen wird ebensowenig durch Vitamin-A-Säure beeinflußt wie die DNA-Polymerase-Aktivität isolierter Zellkerne. Dagegen lassen sich Einflüsse der Vitamin-A-Säure auf zellfreie Proteinbiosynthese-Systeme, sowie auf die RNA-Polymerasen von Tumorzellkernen nachweisen. Es wird diskutiert, daß für die primären, starken Hemmeffekte auf Ehrlich-Ascites-Tumorzellen in-vitro, die schon bekannten lysosomen-labilisierenden Eigenschaften der Vitamin-A-Säure verantwortlich sind.

Investigations on the cytostatic mechanism of vitamin-A-acid

Summary

The cytostatic effects of vitamin-A-acid on Ehrlich-ascites tumor cells in-vitro and on some cell-free enzyme systems were investigated. In the presence of various vitamin-A-acid concentrations the rates of 14C-thymidine-, -uridine- and -leucine-incorporation were progressively diminished in an unspecific way. Neither partially purified thymidine kinase nor the DNA polymerase activity of isolated nuclei proved to be affected by vitamin-A-acid. In contrast, some influence on the cell-free systems of protein biosynthesis and on the tumor nuclei RNA polymerases could be shown. It is discussed, that the known lysosome-labilizing properties of vitamin-A-acid are responsible for the primary strong inhibitory effects on Ehrlich ascites tumor cells in-vitro.

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • A. Jung
    • 1
  • E. Bauer
    • 1
  • K. Lenger
    • 1
  • A. Scholz
    • 1
  • R. Jackisch
    • 1
  1. 1.Physiologisch-Chemisches Institut der UniversitätTübingen

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