Theoretical and Applied Genetics

, Volume 75, Issue 2, pp 286–290

Location of β-amylase sequences in wheat and its relatives

  • P. J. Sharp
  • M. Kreis
  • P. R. Shewry
  • M. D. Gale
Article

DOI: 10.1007/BF00303966

Cite this article as:
Sharp, P.J., Kreis, M., Shewry, P.R. et al. Theoret. Appl. Genetics (1988) 75: 286. doi:10.1007/BF00303966

Summary

A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtained from wheat aneuploid and wheat-alien addition lines. Structural genes were identified on homoeologous group 4 and 5 chromosomes, confirming the results of isozyme studies. In addition, a further set of structural genes was found on homoeologous group 2 chromosomes. It is proposed that there are two homoeoallelic series, β-Amy-1 on group 4 or 5 chromosomes, and β-Amy-2 on group 2 chromosomes. Evidence is presented that each locus contains one or two β-amylase structural genes, and it is suggested that the large number of isozymes seen upon IEF are due to post-translational modifications.

Keywords

RFLP Wheat Aegilops Rye β-Amylase 

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • P. J. Sharp
    • 1
  • M. Kreis
    • 2
  • P. R. Shewry
    • 2
  • M. D. Gale
    • 1
  1. 1.AFRC Institute of Plant Science Research, Plant Breeding InstituteTrumpingtonUK
  2. 2.AFRC Institute of Arable Crops Research, Rothamsted Experimental StationHarpendenUK

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