Homologies to chloroplast DNA in the nuclear DNA of a number of Chenopod species
Sequences homologous to chloroplast (ct)DNA have been found in nuclear DNA in five species of the Chenopodiaceae, extending the earlier observations of ‘promiscuous’ DNA in Spinacia oleracea (Timmis and Scott 1983). Using the 7.7 kbp spinach ctDNA Pst I fragment as a hybridization probe, several separately located homologies to ctDNA were resolved in the nuclear DNA of Beta vulgaris, Chenopodium quinoa, and Enchylaena tomentosa. In Chenopodium album and Atriplex cinerea the major region of homology was to a nuclear Eco RI fragment (6 kbp) indistinguishable from that in ctDNA. These homologies may therefore involve larger tracts of ctDNA because the same restriction sites are apparently retained in the nucleus. This suggests that in these latter two species there is a contrasting, more homogeneous arrangement of ctDNA transpositions in the nucleus.
Key wordsChloroplast DNA Nuclear DNA DNA homologies Chenopods
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- Dyer TA (1984) In: Baker NR, Barber J (eds) The chloroplast genome: its nature and role in development in chloroplast biogenesis. Elsevier, Amsterdam, pp 23–69Google Scholar
- Gruenbaum Y, Naveh-Many T, Cedar H, Razin A (1981) Sequence specificity of methylation in higher plant DNA. Nature 292:860–862Google Scholar
- Palmer JD, Thompson WF (1981) Clone banks of mungbean, pea and spinach chloroplast genomes. Gene 15:21–26Google Scholar
- Sano H, Grabony C, Sager R (1984) Loss of chloroplast DNA methylation during dedifferentiation of Chlamydomonas reinhardi gametes. Mol Cell Biol 4:2103–2108Google Scholar
- Scott NS, Possingham JV (1980) Chloroplast DNA in expanding spinach leaves. J Exp Bot 123:1081–1092Google Scholar
- Scott NS, Timmis JN (1984) Homologies between nuclear and plastid DNA in spinach. Theor Appl Genet 67:279–288Google Scholar
- Smillie RM, Scott NS (1969) Organelle biosynthesis: the chloroplast. In: Progress in molecular and subcellular biology, vol I. Springer, Berlin Heidelberg New York, pp 136–202Google Scholar
- Timmis JN, Scott NS (1983) Spinach nuclear and chloroplast DNAs have homologous sequences. Nature 305:65–67Google Scholar
- Timmis JN, Scott NS (1984) Promiscuous DNA: sequence homologies between DNA of separate organelles. Trends Biochem Sci 9:271–273Google Scholar