Archives of Microbiology

, Volume 161, Issue 4, pp 328–332 | Cite as

Desulfuromonas acetexigens sp. nov., a dissimilatory sulfur-reducing eubacterium from anoxic freshwater sediments

  • Kai Finster
  • Friedhelm Bak
  • Norbert Pfennig
Original Papers


Five strains of obligate anaerobic sulfur-reducing eubacteria that exclusively use acetate as energy and carbon source have been enriched and isolated from anoxic sulfide-containing freshwater mud. The strains were unable to grow in the presence of 2% NaCl. Morphologically the strains were not uniform, cells were either rod-shaped or elongated ovoid. All strains were flagellated with a single polar to subpolar flagellum. They stained gram-negative. Two of the strains were studied in detail. Malate or fumarate was used alternatively to elemental sulfur as electron acceptor. The capacity to grow on acetate as sole organic substrate and to reduce elemental sulfur or polysulfide to sulfide are traits in common with the genus Desulfuromonas. The strains differ from Desulfuromonas acetoxidans by their freshwater origin, morphology, metabolic specialization and their DNA base ratio. Therefore we consider the new isolates as a new species for which the name Desulfuromonas acetexigens is proposed.

Key words

Desulfuromonas acetexigens (description) Reduction of elemental sulfur/polysulfide Anoxic freshwater mud Anaerobic acetate oxidation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bonch-OsmolovskayaEA, SokolovaTG, KostrikinaNA, ZavarzinGA (1990) Desulfurella acetivorans gen. nov. and sp. nov. a new thermophilic sulfur-reducing eubacterium. Arch Microbiol 153: 151–155Google Scholar
  2. BoulegueJ (1978) Solubility of elemental sulfur in water at 298 K. Phosphorus Sulfur 5: 127–128Google Scholar
  3. FowlerVJ, WiddelF, PfennigN, WoeseCR, StackebrandtE (1986) Phylogenetic relationship sulfate- and sulfur-reducing eubacteria. Syst Appl Microbiol 8: 32–41Google Scholar
  4. HeijtuisenJHFG, HansenTA (1989) Betaine fermentation and oxidation by marine Desulfuromonas strains. Appl. Environ Microbiol 55: 965–969Google Scholar
  5. LaanbroekHJ, PfennigN (1981) Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and marine sediments. Arch Microbiol 128: 330–335Google Scholar
  6. LaanbroekHJ, StalLJ, VeldkampH (1978) Utilization of hydrogen and formate by Campylobacter spec under aerobic and anaerobic conditions. Arch Microbiol 119: 99–102Google Scholar
  7. MarmurJ, DotyP (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5: 109–118Google Scholar
  8. PfennigN (1978) Rhodocyclus purpureus gen. nov. and sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28: 283–288Google Scholar
  9. PfennigN, BieblH (1976) Desulfuromonas acetoxidans gen. nov. and sp. nov., a new anaerobic, sulfur-reducing, acetate-oxidizing bacterium. Arch Microbiol 110: 3–12Google Scholar
  10. SchuhmacherW, KroneckPMHG, PfennigN (1992) Comparative systematic study on “Spirillum” 5175, Campylobacter and Wolinela species. Description of “Spirillum” 5175 as Sulfurospirillum deleyianum gen. nov. and sp. nov. Arch Microbiol 158: 287–293Google Scholar
  11. WiddelF (1988) Microbiology and ecology of sulfate- and sulfur-reducing bacteria. In: ZehnderAJB (ed) Biology of anaerobic microorganisms. Wiley, New York, pp 469–585Google Scholar
  12. WiddelF, HansenTA (1992) The dissimilatory sulfate- and sulfur-reducing bacteria. In: BalowsA, TrüperHG, DworkinM, HarderW, SchleiferKH (eds) the prokaryotes, 2nd edn, vol 1. Springer, New York Berlin Heidelberg, pp 583–624Google Scholar
  13. WiddelF, PfennigN (1977) A new anaerobic, sporing acetate-oxidizing, sulfate-reducing bacterium, Desulfotomaculum (emend.) acetoxidans. Arch Microbiol 112: 119–122Google Scholar
  14. WiddelF, PfennigN (1984) Dissimilatory sulfate- and sulfur-reducing bacteria. In: HoltJG, KriegNR (eds) Bergey's manual of systematic bacteriology, vol. 1. Williams and Wilkins, Baltimore London, pp 663–679Google Scholar
  15. WiddelF, PfennigN (1992) The genus Desulfuromonas and other gram-negative sulfur-reducing eubacteria. In: BalowsA, TrüperHG, DworkinM, HarderW, SchleiferKH (eds) The prokaryotes, 2nd edn, vol 4. Springer, New York Berlin Heidelberg, pp 3379–3389Google Scholar
  16. WolfeRS, PfennigN (1977) Reduction of sulfur by Spirillum 5175 and syntrophism with Chlorobium. Appl Environ Microbiol 33: 427–433Google Scholar
  17. ZinderSH, BrockTD (1978) Dimethyl sulfoxide as an electron acceptor for anaerobic growth. Arch Microbiol 116: 35–40Google Scholar

Copyright information

© Springer Verlag 1994

Authors and Affiliations

  • Kai Finster
    • 1
  • Friedhelm Bak
    • 2
  • Norbert Pfennig
    • 3
  1. 1.Institute of Biological Sciences, Department of Microbial EcologyUniversity of ÅrhusÅrhus CDenmark
  2. 2.Max-Planck-Institut für Terrestrische MikrobiologieMarburgGermany
  3. 3.ÜberlingenGermany

Personalised recommendations