Archives of Toxicology

, Volume 44, Issue 1–3, pp 23–30 | Cite as

Are findings with isolated rat livers after short calcium free perfusion relevant for isolated cells?

  • H. Höke
  • H. Krell
  • E. Pfaff


In the isolated liver transient perfusion without calcium results in cholestasis which was characterized by an increased efflux rate across the sinusoidal membrane and inhibition of the concentrative transport of bromosulfophthalein to the canalicular side of the cell. Cholestasis could not be reversed within the usual duration of liver perfusion.

Key words

Isolated perfused liver Isolated liver cells Bile secretion BSP (bromosulfophthalein) Calcium 


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  1. Baur, H., Kasperek, S., Pfaff, E.: Criteria of viability of isolated liver cells. Hoppe-Seylers Z. Physiol. Chem. 356, 827–838 (1975)Google Scholar
  2. Berry, M. N., Friend, D. S.:High-yield preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43, 506–520 (1969)Google Scholar
  3. Dammann, H.-G.: Determination of the biliary tree capacity of the rat in its distended and nondistended state using 35S-sulfobromophthalein glutathione (35S-BSP-GSH) as a marker. J. Physiol. (Paris) 73, 37–46 (1977)Google Scholar
  4. Graf,J.,Kaschnitz,R.,Korn,P.,Peterlik, M.:Dependence of bile formation on calcium. Digestion 8, 113 (1973)Google Scholar
  5. Krell, H., Höke, H., Pfaff, E.: Studies on the dependence of bile flow on extracellular Ca2+. Naunyn-Schmiedeberg's Arch. Pharmacol. 297 (Suppl.), R42 (1977)Google Scholar
  6. Owen, C. A.: Isolated rat liver needs calcium to make bile. Proc. Soc. Exp. Biol. Med. 155, 314–317 (1977)Google Scholar
  7. Scholz, R., Hanson, W., Thurman, R. G.: Interaction of mixed-function oxidation with biosynthetic processes. I. Inhibition of gluconeogenesis by aminopyrine in perfused rat liver. Eur. J. Biochem. 38, 64–72 (1973)Google Scholar
  8. Schwarz, L. R., Burr, R., Schwenk, M., Pfaff, E., Greim, H.:Uptake of taurocholic acid into isolated rat liver cells. Eur. J. Biochem. 55, 617–623 (1975)Google Scholar
  9. Schwarz, L. R., Schwenk, M., Pfaff, E., Greim, H.: Excretion of taurocholate from isolated hepatocytes. Eur. J. Biochem. 71, 369–373 (1976)Google Scholar
  10. Schwarz, L. R., Summer, K. H., Schwenk, M.: Transport and metabolism of bromosulfophthalein by isolated rat liver cells. Eur. J. Biochem. 94, 617–622 (1979)Google Scholar
  11. Schwenk, M., Burr, R., Schwarz, L. R., Pfaff, E.: Uptake of bromosulfophthalein by isolated liver cells. Eur. J. Biochem. 64, 189–197 (1976)Google Scholar
  12. Sies, H., Summer, K. H.:Hydroperoxide-metabolizing systems in rat liver. Eur. J. Biochem. 57, 503–512 (1975)Google Scholar
  13. Sies, H.,Wahlländer,A.,Linke,I.,Marklstorfer,A.:Glutathione disulfide (GSSG) efflux from liver occurs via excretion into bile. Hoppe-Seylers Z. Physiol. Chem. 359, 1151–1152 (1978)Google Scholar
  14. Tietze,F.:Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem. 27, 502–522 (1969)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • H. Höke
    • 1
  • H. Krell
    • 1
  • E. Pfaff
    • 1
  1. 1.Institut für Toxikologie der Universität TübingenTübingenFederal Republic of Germany

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