Advertisement

Cell and Tissue Research

, Volume 277, Issue 1, pp 115–121 | Cite as

Distribution of collagens type I, type III and type V in the pancreas of rat, dog, pig and man

  • J. H. M. Van Deijnen
  • P. T. R. Van Suylichem
  • G. H. J. Wolters
  • R. Van Schilfgaarde
Article

Abstract

The presence of collagens type I, type III and type V was determined immunohistochemically in pancreatic tissue of rat, pig, dog and man. The reaction to anti-collagen type I is weak (pig, dog) or moderate (rat, man) in the peri-insular region and in the lobar, lobular and acinar septa, whereas the reaction to anti-collagen type III is well developed. In rat and dog, the latter reaction deposit on the lobar and acinar septa is prominent. These elements only show a moderate reaction intensity in pig and man. The peri-insular region displays a weak (rat, dog, man) or very weak (pig) reaction against collagen type III. Anti-collagen type V reacts moderately (rat, dog, man) or weakly (pig) in the lobar and lobular septa. The acinar septa show a moderate (rat, dog, man) or very weak (pig) reaction. This information regarding the types and distribution of the collagenous compounds in pancreatic extracellular matrix could lead to differentiated enzymatic pancreas dissociation and, ultimately, increased islet yield and improved reproducibility of pancreatic islet isolation procedures for transplantation purposes.

Key words

Collagen type I Collagen type III Collagen type V Pancreas dissociation Islet isolation Dog Rat (Wistar) Pig Man 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bond MD, Van Wart HE (1984) Characterization of the individual collagenases from Clostridium histolyticum. Biochemistry 23:3085–3091Google Scholar
  2. French MF, Bhown A, Van Wart HE (1992) Identification of Clostridium histolyticum collagenase hyperreactive sites in type I, II, and III collagens: lack of correlation with triple helical stability. J Prot Chem 11:83–97Google Scholar
  3. Hawkes T, Nidag E, Gordon J (1982) A dot immunobinding assay for monoclonal and other antibodies. Anal Biochem 119:142–147Google Scholar
  4. Hefley T, Cushing J, Brand JS (1981) Enzymatic isolation of cells from bone: cytotoxic enzymes of bacterial collagenase. Am J Physiol 240:C234–C238Google Scholar
  5. Hefley TJ (1987) Utilization of FPLC-purified bacterial collagenase for the isolation of cells from bone. J Bone Mineral Res 2:505–516Google Scholar
  6. Kafatos FC, Jones CW, Efstratiadis A (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res 7:1541Google Scholar
  7. Kennedy RH, Bockman DE, Uscanga L, Choux R, Grimaud J-A, Sarles H (1987) Pancreatic extracellular matrix alterations in chronic pancreatitis. Pancreas 2:61–72Google Scholar
  8. Kneteman NM, warnock GL, Evans MG, Nason RW, Rajotte RV (1990) Prolonged function of canine pancreatic fragments autotransplanted to the spleen by venous reflux. Tranplantation 49:679–681Google Scholar
  9. López de León A, Rojkind M (1985) A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J Histochem Cytochem 33:737–743Google Scholar
  10. Mallya SK, Mookhtiar KA, Van Wart HE (1992) Kinetics of hydrolysis of type I, II, and III collagens by the class I and II Clostridium histolyticum collagenases. J Prot Chem 11:99–107Google Scholar
  11. Martinez-Hernandez A (1987) Electron immunohistochemistry of the extra-cellular matrix: an overview. Enzymology 145:78–102Google Scholar
  12. Matrisian LM (1990) Metalloproteinases and their inhibitors in matrix remodeling. Trends Genet 6:121–125Google Scholar
  13. Miller EJ, Rhodes RK (1982) Preparation and characterization of the different types of collagen. Methods Enzymol 82:33–64Google Scholar
  14. Scharp DW, Lacy PE, Santiago JV, McCullough CS, Weide LG, Falqui L, Marchetti P, Gingerich RL, Jaffe AS, Cryer PE, Anderson CB, Flye MW (1990) Insulin independence after islet transplantation into type I diabetic patient. Diabetes 39:515–518Google Scholar
  15. Sternberger LA (1979) Immunocytochemistry, 2nd edn. Wiley, New York, pp 104–169Google Scholar
  16. Sykes B, Puddle B, Francis M, Smith R (1976) The estimation of two collagens from human dermis by interrupted gel electrophoresis. Biochem Biophys Res Commus 72:1472–1480Google Scholar
  17. Tzakis AG, Ricordi C, Alejandro R, Zeng Y, Fung JJ, Todo S, Demetris AJ, Mintz DH, Starzl TE (1990) Pancreatic islet transplantation after upper abdominal exenteration and liver replacement. Lancet 336:402–405Google Scholar
  18. Uscanga L, Kennedy RH, Stoker G, Grimaud J-A, Sarles H (1984) Immunolocalization of collagen types, laminin and fibronectin in normal human pancreas. Digestion 30:158–164Google Scholar
  19. Uscanga L, Kennedy RH, Choux R, Deuguet M, Grimaud J-A, Sarles H (1987) Sequential connective matrix changes in experimental acute pancreatitis. An immunohistochemical and biochemical assessment in the rat. Int J Pancreatol 2:33–45Google Scholar
  20. Van Deijnen JHM, Hulstaert CE, Wolters GHJ, Van Schilfgaarde R (1992) Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man. Cell Tissue Res 267:139–146Google Scholar
  21. Van Suylichem PTR, Pasma A, Wolters GHJ, Van Schilfgaarde R (1987) Microscopic aspects of the structure and collagen content of the pancreas from the perspective of islet isolation. Transplant Proc 19:3958–3959Google Scholar
  22. Warnock GL, Rajotte RV (1988) Critical mass of purified islets that induce normoglycemia: after implantation into dogs. Diabetes 37:467–470Google Scholar
  23. Warnock GL, Kneteman NM, Ryan E, Evans MG, Seelis REA, Halloran PF, Rabinovitch A, Rajotte RV (1989) Continued function of pancreatic islets after transplantation in type I diabetes. Lancet II:570–572Google Scholar
  24. warnock GL, Kneteman NM, Ryan E, Seelis REA, Rabinovitch A, Rajotte RV (1991) Normoglycemia after transplantation of freshly isolated and cryopreserved pancreatic islets in type I (insulin-dependent) diabetes mellitus. Diabetologia 34:55–58Google Scholar
  25. Wolters GHJ, Van Suylichem PTR, Van Deijnen JHM, Van Schilfgaarde R (1990) Factors influencing the isolation process of islets of Langerhans. Horm Metab Res [Suppl] 25:20–26Google Scholar
  26. Wolters GHJ, Vos-Scheperkeuter GH, Van Deijnen JHM, Van Schilfgaarde R (1992) An analysis of the role of collagenase and protease in the enzymic dissociation of the rat pancreas for islet isolation. Diabetologia 35:735–742Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. H. M. Van Deijnen
    • 1
  • P. T. R. Van Suylichem
    • 1
  • G. H. J. Wolters
    • 1
  • R. Van Schilfgaarde
    • 1
  1. 1.Surgical Research Laboratory, Department of SurgeryUniversity of GroningenGroningenThe Netherlands

Personalised recommendations