Human Genetics

, Volume 60, Issue 3, pp 262–266

Flow cytometric characterization of a Chinese hamster x man hybrid cell line retaining the human Y chromosome

  • C. Cremer
  • J. W. Gray
  • H. H. Ropers
Original Investigations

Summary

A Chinese hamster x man hybrid cell line (CH-Y-VII) was established which retains a free human Y chromosome. Exponentially growing CH-Y-VII cells were arrested with colcemid; metaphase chromosomes were isolated and stained with 33258 Hoechst (HO) plus Chromomycin A3 (CA3), or with ethidium bromide (EB). The HO/CA3-stained chromosomes were measured in a dual beam flow cytometer, and bivariate HO/CA3 flow karyotypes and univariate HO and CA3 flow karyotypes were established. EB-stained chromosomes were analyzed in a modified Becton Dickinson FACS-Sorter. For all three stains used, the human Y chromosome forms a separate peak in univariate flow karyotypes; the optimum resolution was obtained for the HO distribution. In the bivariate HO/CA3 flow karyotype, the peak for the human Y chromosome is completely separated from the Chinese hamster chromosomes.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • C. Cremer
    • 1
  • J. W. Gray
    • 2
  • H. H. Ropers
    • 1
  1. 1.Institute of Human GeneticsUniversity of FreiburgFreiburgFederal Republic of Germany
  2. 2.Biomedical Sciences Division, Lawrence Livermore National LaboratoryUniversity of CaliforniaLivermoreUSA

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