Cell and Tissue Research

, Volume 267, Issue 2, pp 267–272 | Cite as

Atrial natriuretic peptide (ANP): a study of ANP and its mRNA in cardiocytes, and of plasma ANP levels in non-obese diabetic mice

  • Hiroharu Mifune
  • Syusaku Suzuki
  • Junichi Honda
  • Yuta Kobayashi
  • Yasutaka Noda
  • Yoshihiro Hayashi
  • Koshi Mochizuki
Article
Received:
Accepted:

Summary

Atrial natriuretic peptide (ANP) levels in cardiocytes and plasma were examined by using immunohistochemistry, electron microscopy, and radioimmunoassay in non-obese diabetic mice (NOD). Cardiocyte ANP mRNA expression was measured by the polymerase chain reaction method. ANP immunoreactivity in the auricular cardiocytes was more prominent in hyperglycemic mice (NOD-h) than in normoglycemic mice (NOD-n). Ultrastructural examination showed that auricular cardiocytes of the NOD-h group contained more cytoplasmic granules than cells of the NOD-n group. Ultrastructural morphometry indicated that the number of granules per auricular cardiocyte was significantly larger in the NOD-h group than in the NOD-n group. (P<0.01), whereas the granule diameter was significantly smaller in the NOD-h group (P<0.01). Radioimmunoassay showed that ANP levels in the NOD-h auricular cardiocytes were significantly higher than those in the NOD-n cardiocytes (P<0.01); the opposite was true in plasma. Cardiocyte ANP mRNA expression was lower in the NOD-h group than in the NOD-n group.

Key words

Atrial natriuretic peptides mRNA Diabetes, type I Immunohistochemistry Morphometry Mouse (NOD) 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bold de AJ (1979) Heart atria granularity effects of changes in water-electrolyte balance. Proc Soc Exp Biol Med 161:508–511Google Scholar
  2. Bold de AJ (1982) Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules. Can J Physiol Pharmacol 60:324–330Google Scholar
  3. Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, Chapeau C, Garcia P, Hamet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry 80:113–127Google Scholar
  4. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159Google Scholar
  5. Christlieb AR, Kaldany A, D'Elia JA (1976) Plasma renin activity and hypertension in diabetes mellitus. Diabetes 25:969–974Google Scholar
  6. Feldt-Rasmussen B, Mathiesen ER, Deckert T, Giese J, Christensen NJ, Bent-Hansen L, Nielsen MD (1987) Centrla role for sodium in the pathogenesis of blood pressure changes independent of angiotensin, aldosterone and catecholamines in type I (insulin-dependent) diabetes mellitus. Diabetologia 30:610–617Google Scholar
  7. Fujita T, Yui R, Kusumoto Y, Serizawa Y, Makino S, Tochino Y (1982) Lymphocytic insulitis in a ‘non-obese diabetic (NOD)’ strain of mice: an immunohistochemical and electron microscope investigation. Biomed Res 3:429–443Google Scholar
  8. Fushimi H, Nonaka K, Tarui S, Tochino Y, Kanaya H (1980) The effects of parabiosis on serum and kidney glycosidase activities in spontaneously diabetic mice. Diabetologia 19:50–53Google Scholar
  9. Gall JAM, Alcorn D, Fernley R, Coghlan JP, Ryan GB (1990) Qualitative and quantitative analysis of granules in atrial appendage cardiocytes in different physiological states. Cell Tissue Res 259:529–534Google Scholar
  10. Jungmann E, Walter-Schrader MC, Haak T, Fassbinder W, Wambach G, Althoff PH, Schoffling K (1988) Impaired renal responsiveness to human atrial natriuretic peptide (hANP) in normotensive patients with type I diabetes mellitus. Klin Wochenschr 66:527–532Google Scholar
  11. Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun 118:131–139Google Scholar
  12. Kangawa K, Fukuda A, Matsuo H (1985) Structural identification of β- and γ-human atrial natriuretic polypeptides. Nature 313:397–400Google Scholar
  13. Kimura T, Abe K, Ota K, Omata K, Shoji M, Kubo K, Matsui K, Inoue M, Yasujima M, Yoshinaga K (1986) Effects of acute water load, hypertonic saline infusion, and furosemide administration on atrial natriuretic peptide and vasopressin release in humans. J Clin Endocrinol Metab 62:1003–1010Google Scholar
  14. Makino S, Kunimoto K, Muraoka Y, Katagiri K (1980) Breeding of a non-obese, diabetic strain of mice. Exp Anim 29:1–13Google Scholar
  15. Mifune H, Suzuki S, Noda Y, Mohri S, Mochizuki K (1991a) Fine structure of atrial natriuretic peptide (ANP)-granules in the atrial cardiocytes of the mouse, rat and Mongolian gerbil. Exp Anim 40:183–193Google Scholar
  16. Mifune H, Suzuki S, Noda Y, Mohri S, Mochizuki K (1991b) Fine structure of atrial natriuretic peptide (ANP)-granules in the cardiocytes in the pig, cattle and horse. J Vet Med Sci 53:561–568Google Scholar
  17. Nakao K, Sugawara A, Morii N, Sakamoto M, Suda M, Soneda J, Ban T, Kihara M, Yamori Y, Shimokura M, Kiso Y, Imura H (1984) Radioimmunoassay for α-human and rat atrial natriuretic polypeptide. Biochem Biophys Res Commun 124:815–821Google Scholar
  18. Nakayama K, Ohkubo H, Hirose T, Inayama S, Nakanishi S (1984) mRNA sequence for human cardiodilatin-atrial natriuretic factor precursor and regulation of precursor mRNA in rat atria. Nature 23:699–701Google Scholar
  19. O'Hare JP, Roland JM, Walters G, Corrall RJM (1986) Impaired sodium excretion in response to volume expansion induced by water immersion in insulin-dependent diabetes mellitus. Clin Sci 71:403–409Google Scholar
  20. Ohneda A, Kobayashi T, Nihei J, Tochino Y, Kanaya H, Makino S (1984) Insulin and glucagon in spontaneously diabetic nonobese mice. Diabetologia 27:460–463Google Scholar
  21. Sagnella GA, Markandu ND, Shore AC, MacGregor GA (1987) Plasma immunoreactive atrial natriuretic peptide and changes in dietary sodium intake in man. Life Sci 40:139–143Google Scholar
  22. Schwartz D, Katsube NC, Needleman P (1986) Atriopeptin release in conditions of altered salt and water balance in the rat. Biochem Biophys Res Commun 137:922–928Google Scholar
  23. Seidman CE, Block KD, Klein KA, Smith JA, Seidman JG (1984) Nucleotide sequences of the human and mouse atrial natriuretic factor gene. Science 226:1206–1209Google Scholar
  24. Stein BA, Katzeff I, Norton G, DeWet G, Rosendorff C (1990) Differental size distribution of atrial dense granules in spontaneously hypertensive, Wistar-Kyoto and Wistar rats. Acta Anat 137:331–335Google Scholar
  25. Takayanagi R, Tanaka I, Maki M, Inagami T (1985) Effects of changes in water-sodium balance on levels of atrial natriuretic factor messenger RNA and peptide in rats. Life Sci 36:1843–1848Google Scholar
  26. Thibault G, Garcia R, Cantin M, Genest J (1983) Atrial natriuretic factor. Characterization and partial purification. Hypertension 5 [Suppl I]:75–80Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Hiroharu Mifune
    • 1
  • Syusaku Suzuki
    • 2
  • Junichi Honda
    • 3
  • Yuta Kobayashi
    • 4
  • Yasutaka Noda
    • 1
  • Yoshihiro Hayashi
    • 5
  • Koshi Mochizuki
    • 6
  1. 1.Institute of Animal ExperimentKurume University School of MedicineFukuokaJapan
  2. 2.Institute of Laboratory Animal Sciences, Faculty of MedicineKagoshima UniversityKagoshimaJapan
  3. 3.Department of Internal MedicineKurume University School of MedicineFukuokaJapan
  4. 4.Department of PharmacologyShimane Medical UniversityShimaneJapan
  5. 5.Laboratory of Veterinary Anatomy, Faculty of AgricultureUniversity of TokyoTokyoJapan
  6. 6.Laboratory of Biomedical Science, College of Agriculture and Veterinary MedicineNihon UniversityKanagawaJapan

Personalised recommendations