The histochemistry of the spiral prominence
- 54 Downloads
- 5 Citations
Summary
In order to demonstrate the existence of oxidative enzymes, lysosomal enzymes, adenosine triphosphatase, butyrylcholinesterase, esterases and alkaline phosphatase, the spiral prominence and the neighbouring tissues of the guinea pig were studied histochemically. The results were discussed in connection with the function of the spiral prominence. Morphological features and enzymatic activities suggested that the spiral prominence serves as a balance between the perilymph and the endolymph, which are both absorptive and secretory and are depending on chemical and physical conditions.
Keywords
Public Health Enzyme Enzymatic Activity Physical Condition AdenosineZusammenfassung
Die Prominentia spiralis and das umgebende Gewebe werden histochemisch untersucht, um die oxydativen and lysosomalen Fermente, die ATPase, die Butyrylcholinesterase, unspezifische Esterasen und alkalische Phosphatase nachzuweisen. Die Ergebnisse werden in Zusammenhang mit der Funktion der Prominentia spiralis diskutiert.
Die morphologischen Eigenschaften und die Enzymaktivitäten deuten darauf hin, daß die Prominentia spiralis dem Ausgleich zwischen Perilymphe und Endolymphe dient und zwar sowohl absorbierend wie such sezernierend, abhängig von den chemischen and physikalischen Bedingungen.
Preview
Unable to display preview. Download preview PDF.
References
- Balogh, K., Jr.: Decalcification with versene for histochemical study of oxydative enzyme systems. J. Histochem. Cytochem. 10, 232–233 (1962).Google Scholar
- Barka, T., Anderson, P. J.: Histochemical methods for acid phosphatase using hexazonium pararosanilin as coupler. J. Histochem. Cytochem. 10, 741–753 (1962).Google Scholar
- Borghosan, E.: Modality of the cochlear humoral circulation. Laryngoscope (St. Louis) 67, 1266–1285 (1957).Google Scholar
- Burstone, M. S.: Histochemical demonstration of cytochrome oxidase with new amine reagents. J. Histochem. Cytochem. 8, 63–70 (1960).Google Scholar
- Cimino, A., Grisanti, G.: The embryological development of the limbus and of the lateral and anterior wall of the ductus cochlearis in the rabbit. Acta oto-laryng. (Stockh.) Suppl. 223 (1967).Google Scholar
- Duvall, A. J.: The ultrastructure of the external sulcus in the guinea pig cochlear duct. Laryngoscope (St. Louis) 79, 1–29 (1969).Google Scholar
- Fieandt, H. v., Saxén, A.: Struktur und Funktion der Region des Sulcus spiralis externus im Innenohr des Menschen und des jungen Hundes. Z. Anat. Entwickl.Gesch. 106, 534–552 (1937).Google Scholar
- —, — Beiträge zur Histologie der Stria Vascularis und der Prominentia spiralis bei Säugern (Hund und Mensch). Z. Anat. Entwickl.-Gesch. 106, 424–446 (1937).Google Scholar
- Hayashi, M.: Histochemical demonstration of N-acetyl-β-glucosaminidase employing naphthol AS-BI N-acetyl-β-glucosaminide as substrate. J. Histochem. Cytochem. 13, 355–360 (1965).Google Scholar
- — Nakajima, Y., Fishman, W. H.: The cytologic demonstration of β-glucuronidase employing naphthol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report. J. Histochem. Cytochem. 12, 293–297 (1964).Google Scholar
- Ilberg, C. v., Spoendlin, H., Vosteen, K. H.: Die Ultrastruktur und Funktion des Sulcus Spiralis Externus und der Prominentia Spiralis der Meerschweinchenschnecke. Darstellung mittels Thorotrast. Arch. klin. exp. Ohr.-, Nas.- u. Kehlk.-Heilk. 192, 124–136 (1968).Google Scholar
- Ishii, D., Takahashi, T., Balogh, K., Jr.: Glycogen in the inner ear after acoustic stimulation. Acta oto-laryng. (Stockh.) 67, 573–582 (1969).Google Scholar
- Ishii, T., Balogh, K., Jr.: Acid phosphatase activity in the inner ear. Acta otolaryng. (Stockh.) 62, 185–192 (1966).Google Scholar
- — Ishii, D., Balogh, K., Jr.: Lysosomal enzymes in the inner ears of kanamycin treated guinea pigs. Acta oto-laryng. (Stockh.) 65, 449–458 (1968).Google Scholar
- — Murakami, Y., Kimura, R. S., Balogh, K., Jr.: Electron microscopic and histochemical identification of lipofuscin in the human inner ear. Acta oto-laryng. (Stockh.) 64, 17–29 (1967).Google Scholar
- Iwata, N.: Über das Wurzelepithel des Ligamentum spirale der Schnecke. Folia anat. jap. 3, 37–85 (1925).Google Scholar
- Karnovsky, M. J., Roots, L.: A “Direct-Coloring” thiocholine method for cholinesterases. J. Histochem. Cytochem. 12, 219–221 (1964).Google Scholar
- Kirikae, I., Nomura, Y., Hiraide, F.: The capillary in the human cochlea. Acta oto-laryng. (Stockh.) 67, 1–8 (1969).Google Scholar
- Lawrence, M.: Structures of the spiral prominence and external sulcus and their relation to the organ of Corti. Laryngoscope (St. Louis) 66, 796–809 (1956).Google Scholar
- — Histological evidence for localized radial flow of endolymph. Arch. Otolaryng. 83, 406–412 (1966).Google Scholar
- Nomura, Y., Hiraide, F.: Cochlear blood vessel. A histochemical method of its demonstration. Arch. Otolaryng. 88, 231–237 (1968).Google Scholar
- —, — The capillary in the human vestibular labyrinth. A histochemical staining technique. Ann. Otol. (St. Louis) 78, 1220–1226 (1969).Google Scholar
- Saxén, A.: Some observations on the physiology of endolymph stream. Acta oto-laryng. (Stockh.), Suppl. 74, 87–101 (1948).Google Scholar
- — Histological studies of endolymph secretion and resorption in the inner ear. Acta oto-laryng. (Stockh.) 40, 23–31 (1951/1952).Google Scholar
- Shambaugh, G. E.: Über Ban und Funktion des Epithels im Sulcus Spiralis Externus. Z. Ohrenheilk. 58, 280–287 (1909).Google Scholar
- Smith, C. A.: Structure of the stria vascularis and the spiral prominence. Ann. Otol. (St. Louis) 66, 521–536 (1957).Google Scholar
- Spoendlin, H. H.: Spiral prominence. In: Salvatore Iurato et al. Submicroscopic structure of the inner ear, p. 149. Oxford: Pergamon Press 1967.Google Scholar
- Yamamoto, K., Nakai, Y.: Electron microscopic studies on the function of the stria vascularis and the spiral ligament in the inner ear. Ann. Otol. (St. Louis) 73, 332–334 (1964).Google Scholar