Virus Genes

, Volume 3, Issue 1, pp 29–44 | Cite as

Serological probes for some foot-and-mouth disease virus nonstructural proteins

  • Michael Tesar
  • Hans-Gerhard Berger
  • Otfried Marquardt


Foot-and-mouth disease virus (FMDV) O1 Kaufbeuren-specific cDNA fragments were subcloned into the E. coli expression vector pRIT·2T. Fusion proteins thus produced in bacteria were purified by affinity chromatography and inoculated into rabbits. Three sera thus obtained were found to be monospecific for FMDV proteins 3A, 3C, and 3D, respectively. Two others were prevalently directed against protein 2C, but in addition, either to protein 2B or to protein 3A. Five out of six mature nonstructural virus proteins can therefore be separately investigated in FMDV-infected cells, either by indirect immunofluorescence or by radioimmunoprecipitation. Immunofluorescence shows all investigated proteins to be located exclusively in the cytoplasm. One of them, protein 2C, transiently forms aggregates at the periphery of cells. Radioimmunoprecipitation confirmed current knowledge on maturation of FMDV proteins. It was further used to characterize postinfectional sera with regard to FMDV-specific antibodies. Cattle and guinea pig were found to have responded differently to FMDV nonstructural antigens. Furthermore, antigenicity of yet to be described FMDV polypeptides was observed in the guinea pig.

Key words

expression cloning foot-and-mouth disease virus non-structural proteins 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Rueckert R.R. in Fields BN et al. (eds.) Virology. Raven Press, New York, 1985, pp. 705–738.Google Scholar
  2. 2.
    Doel T.R., Sangar D.V., Rowlands D.J. and Brown F., J Gen Virol 41, 395–404, 1978.PubMedGoogle Scholar
  3. 3.
    Grubman M.J., Robertson B.H., Morgan D.O., Moore D.M. and Dowbenko D., J Virol 50, 579–586, 1984.PubMedGoogle Scholar
  4. 4.
    Forss S. and Schaller H., Nucleic Acids Res 10, 6441–6450, 1982.PubMedGoogle Scholar
  5. 5.
    Vakharia V.N., Devaney M.A., Dunn J.J. and Grubman M.J., J Virol 61, 3199–3207, 1987.PubMedGoogle Scholar
  6. 6.
    Harris T.J.R., Brown F. and Sangar D.V., Virology 112, 91–98, 1981.PubMedGoogle Scholar
  7. 7.
    Grubman M.J., Zellner M. and Wagner J., Virology 158, 133–140, 1987.PubMedGoogle Scholar
  8. 8.
    Strebel K., Beck E. Strohmaier K. and Schaller H., J Virol 57, 983–991, 1986.PubMedGoogle Scholar
  9. 9.
    Nilsson B., Abrahmsén L. and Uhlén M., EMBO J 4, 1075–1080, 1985.PubMedGoogle Scholar
  10. 10.
    Küpper H., Keller W., Kurz C., Forss S., Schaller H., Franze R., Strohmaier K., Marquardt O., Zaslavsky V.G. and Hofschneider P.H., Nature 289, 555–559, 1981.PubMedGoogle Scholar
  11. 11.
    Forss S., Strebel K., Beck E. and Schaller H., Nucleic Acids Res 12, 6587–6601, 1984.PubMedGoogle Scholar
  12. 12.
    Klump W., Marquardt O. and Hofschneider P.H., Proc Natl Acad Sci USA 81, 3351–3355, 1984.PubMedGoogle Scholar
  13. 13.
    Lieb M., J Mol Biol 16, 149–163, 1966.PubMedGoogle Scholar
  14. 14.
    Towbin H., Staehlin T. and Gordon J., Proc Natl Acad Sci USA 76, 4350–4354, 1979.PubMedGoogle Scholar
  15. 15.
    Williams C.A. and Chase M.W. in Methods in Immunology and Immunochemistry, Vol. 1, Academic Press, London, 1967, pp. 197–307.Google Scholar
  16. 16.
    Marquardt O. and Adam K.-H., Virus Genes 2, 283–291, 1988.Google Scholar
  17. 17.
    Bonner W.M. and Laskey R.A., Eur J Biochem 46, 83–88, 1974.PubMedGoogle Scholar
  18. 18.
    Rodriguez M., Siegel L.M., Hovanec-Burns D., Bologa L. and Graves M.C., Virology 166, 463–474, 1988.PubMedGoogle Scholar
  19. 19.
    Lutton C.W. and Gauntt C.J., J Virol 60, 294–296, 1986.PubMedGoogle Scholar
  20. 20.
    Bienz K., Egger D. and Pasamontes L., Virology 160, 220–226, 1987.PubMedGoogle Scholar
  21. 21.
    Grigera P.R. and Tisminetzky S.G., Virology 136, 10–19, 1984.PubMedGoogle Scholar
  22. 22.
    Klump W., Soppa J. Marquardt O. and Hofschneider P.H. in Starlinger P. et al. (eds.) 35 Colloquium-Mosbach 1984. Springer Verlag Berlin, Heidelberg, 1985, pp. 202–209.Google Scholar

Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Michael Tesar
    • 1
  • Hans-Gerhard Berger
    • 1
  • Otfried Marquardt
    • 1
  1. 1.Federal Research Center for Virus Diseases of AnimalsTübingenFederal Republic of Germany

Personalised recommendations