Rheumatology International

, Volume 12, Issue 2, pp 53–59

Spontaneous expression of immediately-early response genes c-fos and egr-1 in collagenase-producing rheumatoid synovial fibroblasts

  • A. Trabandt
  • W. K. Aicher
  • R. E. Gay
  • V. P. Sukhatme
  • H. -G. Fassbender
  • S. Gay
Originals

Summary

In view of the important role of interstitial collagenase in the pathogenesis of rheumatoid arthritis (RA), we studied the expression of fibroblast-type collagenase in rheumatoid synovium and searched for its potential transcription factors, namely the oncoprotein c-fos and the early-growth-response gene-1 (egr-1), an inducible zinc-finger encoding gene. Elevated levels of RNA sequences complimentary to c-fos and egr-1 cDNA probes could be detected in cytoplasmic extracts of collagenase-expressing synovial fibroblast-like cells when compared to equivalent RNA amounts isolated from control fibroblasts. Utilizing immunocytochemistry, immunoreactivity for c-fos oncoprotein was found in 13 of 19 joint specimens obtained from patients with active RA. These oncoprotein data were positively correlated to the collagenase expression in the same specimens. Moreover, immunohistochemical analysis confirmed the localization of both oncoprotein c-fos and fibroblast-type collagenase within synovial fibroblast-like cells attached to bone erosions.

Key words

Collagenase c-fos egr-1 Pathogenesis of rheumatoid arthritis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. Trabandt
    • 1
  • W. K. Aicher
    • 2
  • R. E. Gay
    • 1
  • V. P. Sukhatme
    • 3
  • H. -G. Fassbender
    • 4
  • S. Gay
    • 1
  1. 1.Division of Clinical Immunology and Rheumatology, Department of MedicineThe University of Alabama at BirminghamBirminghamUSA
  2. 2.Department of MicrobiologyThe University of Alabama at BirminghamBirminghamUSA
  3. 3.Howard Hughes Medical InstituteUniversity of Chicago, Billings HospitalChicagoUSA
  4. 4.WHO CentreMainzFederal Republic of Germany

Personalised recommendations