Urological Research

, Volume 18, Issue 5, pp 305–308 | Cite as

Secretory immunoglobulin A and inhibitory activity of bacterial adherence to epithelial cells in urine from patients with urinary tract infections

  • A. Trinchieri
  • L. Braceschi
  • D. Tiranti
  • S. Dell'Acqua
  • A. Mandressi
  • E. Pisani
Original Articles

Summary

To assess the role of local immune response against bacterial invasion of the urinary tract we studied 168 patients with bacteriuria. Urinary secretory immunoglobulins A (sIgA) were measured using radial immunodiffusion or enzyme-linked immunosorbent assay (ELISA). In particular, ELISA is a very suitable assay for measuring the low levels of sIgA in urine. Furthermore, we used a quantitative in vitro adherence assay to investigate the attachment of Escherichia coli to human uroepithelial cells after incubation in urine from patients with urinary tract infection. Urine from patients with ileocystoplasty was significantly more potent in inhibiting bacterial adherence than was urine from other groups of patients with urinary tract infection. The presence of high urinary sIgA may help explain the increased antiadherence activity of urine in patients with ileocystoplasty. Mean urinary sIgA in patients with upper urinary tract infection was higher than in patients with uncomplicated infection in the lower urinary tract. Alterations in mucosal immune functions may account for the propensity toward bacterial colonization in women prone to uncomplicated urinary tract infection.

Key words

Secretory immunoglobulin A Bacterial adhesion Urinary tract infection Ileocystoplasty 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fliedner M, Mehls O, Rauterberg EW, Ritz E (1986) Urinary sIgA in children with urinary tract infection. J Pediatr 109:416Google Scholar
  2. 2.
    Hjelm EM (1984) Local cellular immune response in ascending urinary tract infection: occurrence of T-cells, immunoglobulin-producing cells and Ia-expressing cells in rat urinary tract tissue. Infect Immun 44:627Google Scholar
  3. 3.
    Hopkins WJ, Uchling DT, Balish E (1987) Local and systemic antibody responses accompany spontaneous resolution of experimental cystitis in Cynomolgus monkeys. Infect Immun 55:1951Google Scholar
  4. 4.
    Jensen J, Uehling DT, Kim K, Seagren-Rasmunssen K, Balish E (1984) Enhanced immune response in the urinary tract of the rat following vaginal immunization. J Urol 132:164Google Scholar
  5. 5.
    Kallenius G, Winberg J (1978) Bacterial adherence to periurethral epithelial cells in girls prone to urinary-tract infections. Lancet II:540Google Scholar
  6. 6.
    Kaufmann DN, Katz R, McIntosh RN (1970) Secretory IgA in urinary tract infection. Br Med J 4:463Google Scholar
  7. 7.
    Mansson W, Colleen S, Low K, Mardh PA, Lundblad A (1985) Immunoglobulins in urine from patients with ileal and colonic conduits and reservoirs. J Urol 133:713Google Scholar
  8. 8.
    McDermott MR, Bienenstock J (1979) Evidence for a common mucosal immunological system: I. Migration of B immunoblasts into intestinal, respiratory and genital tissues. J Immunol 122:1892Google Scholar
  9. 9.
    Riedasch G, Heck P, Rauterberg E, Ritz E (1983) Dose low urinary sIgA predispose to urinary tract infection? Kidney Int 23:759Google Scholar
  10. 10.
    Savoia D, Martinetto P, Achino A, Pugliese A (1983) Adhesion of Proteus species to various cell types. Eur J Clin Microbiol 2:571Google Scholar
  11. 11.
    Schaeffer AJ, Jones JM, Dunn JK (1981) Association of in vitro Escherichia coli adherence to vaginal and buccal epithelial cells with susceptibility of women to recurrent urinary-tract infections. N Engl J Med 304:1062Google Scholar
  12. 12.
    Silverblatt FJ, Cohen LS (1979) Antipili antibody affords protection against experimental ascending pyelonephritis. J Clin Invest 64:333Google Scholar
  13. 13.
    Svanborg Edén C, Hanson LA, Jodal U, Lindberg U, Sohl Akerlund A (1976) Variable adherence to normal human urinary tract epithelial cells of E. coli strains associated with various forms of urinary tract infections. Lancet II:490Google Scholar
  14. 14.
    Svanborg Edén C, Eriksson B, Hanson LA (1977) Adhesion of Escherichia coli to human uroepithelial cells in vitro. Infect Immun 18:767Google Scholar
  15. 15.
    Szabo RJ, Dairiki Shortliffe LM, Stamey TA (1987) Adherence of Escherichia coli and Proteus mirabilis to human transitional cells. J Urol 137:793Google Scholar
  16. 16.
    Tomasi TB (1972) Secretory immunoglobulins. N Engl J Med 287:500Google Scholar
  17. 17.
    Trinick TR, Laker MF (1984) Measurement of urinary immunoglobulins G, A and M by an enzyme linked immunosorbent assay (ELISA). Clin Chim Acta 139:113Google Scholar
  18. 18.
    Uehling DT, (1973) Immunoglobulin excretion in women with recurrent urinary tract infections. J Urol 109:302Google Scholar
  19. 19.
    Uehling DT, Steihm ER (1971) Elevated urinary secretory IgA in children with urinary tract infection. Pediatrics 47:40Google Scholar
  20. 20.
    Uehling DT, Hopkins WJ, Jensen J, Balish E (1987) Vaginal immunization against induced cystitis in monkeys. J Urol 137:327Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • A. Trinchieri
    • 1
  • L. Braceschi
    • 1
  • D. Tiranti
    • 1
  • S. Dell'Acqua
    • 1
  • A. Mandressi
    • 1
  • E. Pisani
    • 1
  1. 1.Institute of UrologyUniversity of MilanMilanItaly

Personalised recommendations