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Urological Research

, Volume 20, Issue 4, pp 313–316 | Cite as

Ras oncogene point mutations in bladder cancer resistant to cisplatin

  • D. P. WoodJr.
  • A. E. Anderson
  • R. Fair
  • R. S. K. Chaganti
Original Articles

Summary

Bladder tumors respond to cisplatin-based chemotherapy in 68% of cases, but only 30% will have a durable response. Recent studies have suggested that ras point mutations may produce cisplatin drug resistance in cell lines. To determine the role of ras point mutations in human tumors resistant to cisplatin, we evaluated ten tumors exposed to cisplatin and eight untreated bladder tumors for ras point mutations. Using polymerase chain reaction DNA amplification and allele-specific oligohybridization, we found that only one of the ten treated tumors and none of the untreated tumors harbored a ras point mutation. We conclude that ras point mutations occur infrequently in untreated bladder tumors and do not appear to correlate with cisplatin drug resistance in vivo.

Key words

Ras point mutations Cisplatin-resistant bladder cancer 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. P. WoodJr.
    • 1
    • 2
  • A. E. Anderson
    • 1
  • R. Fair
    • 2
  • R. S. K. Chaganti
    • 1
    • 3
  1. 1.Laboratory of Cancer GeneticsSloan-Kettering InstituteNew YorkUSA
  2. 2.Urology Service, Department of Surgery Memorial HospitalMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  3. 3.Department of Pathology, Memorial HospitalMemorial Sloan-Kettering Cancer CenterNew YorkUSA

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