Theoretical and Applied Genetics

, Volume 78, Issue 1, pp 119–128

Genetic diversity in Cucumis sativus L. assessed by variation at 18 allozyme coding loci

  • L. D. Knerr
  • J. E. Staub
  • D. J. Holder
  • B. P. May
Originals

Summary

The genetic diversity of the U.S. Cucumis sativus L. germplasm collection [757 plant introductions (PI) representing 45 countries] was assessed using 40 enzymes which represented 74 biochemical loci. Polymorphisms were observed at 18 loci (G2dh-1, Gpi-1, Gpi-2, Gr-1, Gr-2, Idh, Mdh-1, Mdh-2, Mdh-3, Mpi-2, Pepla-2, Peppap-2, Per-4, Pgd-1, Pgd-2, Pgm-1, Pgm-3, and Skdh). Two PIs (285606 and 215589) contained alleles [G2dh-1(1) and Per-4(2), respectively] which did not occur in any other PI. Other alleles which occurred in low frequencies (in < 1% of the PIs) included Gpi-1(3), Gpi-2(3), Gr-1(3), Gr-2(1), Idh(1), Mdh-1(2), Mdh-2(1), Peppap-2(1), and Pgd-1(1). Individual loci containing more than one allele in greater than 20% of the PIs included Mpi-2, Pepla-2, Pgd-2, and Pgm-1. Multivariate analyses aided in the reduction of data (principle components), depicted relationships among PIs (cluster), and identified the most discriminating enzyme loci (Pgm-1, Pepla-2, Gr-1, Pgd-2, Mpi-2, and Skdh) (classification and regression tree).

Key words

Biochemical loci Cucumber Isozymes Multivariate analysis Starch-gel electrophoresis 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • L. D. Knerr
    • 1
  • J. E. Staub
    • 1
  • D. J. Holder
    • 1
  • B. P. May
    • 2
  1. 1.USDA/ARS, Department of HorticultureUniversity of WisconsinMadisonUSA
  2. 2.Department of Natural ResourcesCornell UniversityIthacaUSA

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