Theoretical and Applied Genetics

, Volume 78, Issue 1, pp 119–128

Genetic diversity in Cucumis sativus L. assessed by variation at 18 allozyme coding loci

  • L. D. Knerr
  • J. E. Staub
  • D. J. Holder
  • B. P. May


The genetic diversity of the U.S. Cucumis sativus L. germplasm collection [757 plant introductions (PI) representing 45 countries] was assessed using 40 enzymes which represented 74 biochemical loci. Polymorphisms were observed at 18 loci (G2dh-1, Gpi-1, Gpi-2, Gr-1, Gr-2, Idh, Mdh-1, Mdh-2, Mdh-3, Mpi-2, Pepla-2, Peppap-2, Per-4, Pgd-1, Pgd-2, Pgm-1, Pgm-3, and Skdh). Two PIs (285606 and 215589) contained alleles [G2dh-1(1) and Per-4(2), respectively] which did not occur in any other PI. Other alleles which occurred in low frequencies (in < 1% of the PIs) included Gpi-1(3), Gpi-2(3), Gr-1(3), Gr-2(1), Idh(1), Mdh-1(2), Mdh-2(1), Peppap-2(1), and Pgd-1(1). Individual loci containing more than one allele in greater than 20% of the PIs included Mpi-2, Pepla-2, Pgd-2, and Pgm-1. Multivariate analyses aided in the reduction of data (principle components), depicted relationships among PIs (cluster), and identified the most discriminating enzyme loci (Pgm-1, Pepla-2, Gr-1, Pgd-2, Mpi-2, and Skdh) (classification and regression tree).

Key words

Biochemical loci Cucumber Isozymes Multivariate analysis Starch-gel electrophoresis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allendorf FW, Mitchell N, Ryman N, Stahl G (1977) Isozyme loci in brown trout (Salmo trutta L.): Detection and interpretation from population data. Hereditas 86:179–190Google Scholar
  2. Ayala FJ, Mourao CA, Perez-Salas S, Richmond R, Dobzhansky T (1970) Enzyme variability in the Drosophila willistoni group. I. Genetic differentiation among sibling species. Proc Natl Acad Sci USA 67:225–232Google Scholar
  3. Barczynska H, Van Klienwee D, Palmer M, Staub JE, Clark R (1988) Evaluation of cucumber germplasm for five pathogens. HortScience 23:778Google Scholar
  4. Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898Google Scholar
  5. Brewer GW (1970) An introduction to isozyme techniques. Academic Press, New YorkGoogle Scholar
  6. Brieman L, Friedman JH, Olshen RA, Stone CJ (1984) Classification and regression trees. Wadsworth, MontereyGoogle Scholar
  7. Chiang YC, Gorman MB, Kiang YT (1987) Inheritance and linkage analysis of phosphoglucose isomerase isozymes in soybeans. Biochem Genet 25:893–900Google Scholar
  8. Clayton JW, Tretiak DN (1972) Amine-citrate buffers for pH control in starch gel electrophoresis. J Fish Res Board Can 29:1169–1172Google Scholar
  9. Crawford DJ (1985) Electrophoretic data and plant speciation. Sys Bot 10:405–416Google Scholar
  10. Cucurbit Genetics Cooperative, Cucurbit Gene List Committee (1985) Gene list for cucumber. Cucurbit Genet Coop Rep 8:86–71Google Scholar
  11. Dane F (1976) Evolutionary studies in the genus Cucumis. PhD Diss, Colorado State University, Fort Collins, 202 ppGoogle Scholar
  12. Dane F (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding Part B. Elsevier, Amsterdam, pp 369–390Google Scholar
  13. Decker DS (1985) Numerical analysis of allozyme variation in Cucurbita pepo. Econ Bot 39:300–309Google Scholar
  14. Esquinas-Alcazar JT (1977) Alloenzyme variation and relationships in the genus Cucumis. PhD Diss, University of California, Davis, 170 ppGoogle Scholar
  15. Fanourakis NE, Simon PW (1987) Analysis of genetic linkage in the cucumber. J Hered 78:238–242Google Scholar
  16. Fedak G (1974) Allozymes as aids to Canadian barley cultivar identification. Euphytica 23:166–173Google Scholar
  17. Goodman MM, Stuber CW (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part B. Elsevier, Amsterdam, pp 369–390Google Scholar
  18. Goodman MM, Stuber CW, Newton K, Weissinger HH (1980) Linkage relationships of 19 enzyme loci in maize. Genet 96:697–710Google Scholar
  19. Harris, RJ (1975) A primer of multivariate statistics. Academic Press, New YorkGoogle Scholar
  20. Hutchins AE (1938) Some examples of heterosis in the cucumber, Cucumis sativus L. Proc Am Soc Hortic Sci 36:660–664Google Scholar
  21. International Board for Plant Genetic Resources (1981) Revised priorities among crops and regions. IBPGR Secretariat, Consultative group on international agricultural research, Rome, 18 ppGoogle Scholar
  22. Kiang YT, Gorman MB (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, Part B. Elsevier, Amsterdam, pp 369–390Google Scholar
  23. Kroon GH, Custers JBM, Kho YO, Den Nijs APM, Varekamp HQ (1979) Interspecific hybridization in Cucumis (L.). I. Need for genetic variation, biosystematic relations and possibilities to overcome crossability barriers. Euphytica 28:723–728Google Scholar
  24. Kupper RS, Staub JE (1988) Combining ability studies between lines of Cucumis sativus L. and Cucumis sativus var. hardwickii (R.) Alef. Euphytica 38:197–216Google Scholar
  25. Markert CL (1975) Isozyme IV. Genetics and evolution. Academic Press, New YorkGoogle Scholar
  26. May B (1980) The Salmonid genome: evolutionary restructuring following a tetraploid event. PhD Thesis, Pennsylvania State UniversityGoogle Scholar
  27. May B, Wright JE, Stoneking M (1979) Joint segregation of biochemical loci in Salmonidae: Results from experiments with Salvelinus and review of the literature on other species. J Fish Res Board Can 36:1114–1128Google Scholar
  28. McLeod MJ, Guttman SI, Eshbaugh WH (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part B. Elsevier, Amsterdam, pp 369–390Google Scholar
  29. Neale DB, Weber JC, Adams WT (1984) Inheritance of needle tissue isozymes in Douglas-fir. Can J Genet Cytol 26:459–468Google Scholar
  30. Orton TJ (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part B. Elsevier, Amsterdam, pp 369–390Google Scholar
  31. Perl-Treves R, Zamir D, Navot N, Galun E (1985) Phylogeny of Cucumis based on isozyme variability and its comparison with plastome phylogeny. Theor Appl Genet 71:430–436Google Scholar
  32. Peterson CE (1975) Plant introduction in the improvement of vegetable cultivars. HortScience 10:575–579Google Scholar
  33. Pollack LM, Gardner CO, Parkhurst AM (1984) Relationships between enzyme marker loci and morphological traits in two mass selected maize populations. Crop Sci 24:1174–1179Google Scholar
  34. Ray AA (1982) SAS Users guide: statistics. SAS Institute, Gary/NCGoogle Scholar
  35. Richmond RC (1972) Enzyme variability in the Drosophila williston group. 3. Amounts of variability in the superspecies D. paulistorum. Genetics 70:87–112Google Scholar
  36. Ridgway GJ, Sherburne SW, Lewis RD (1970) Polymorphism in the esterases of Atlantic herring. Trans Am Fish Soc 99:147–151Google Scholar
  37. Robinson RW, Munger HM, Whitaker TW, Bohn GW (1976) Genes of the Cucurbitaceae. Hortic Sci 11:554–567Google Scholar
  38. Selander RK, Smith MH, Yang SY, Johnson WE, Gentry JB (1971) Biochemical polymorphism and systematics in the genus Peromyseus. I. Variation in the old-field mouse (Peromyseus polionotus). In: Studies in genetics, University of Texas Publication, AustinGoogle Scholar
  39. Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem Genet 4:297–320Google Scholar
  40. Staub JE, Kupper RS, Schuman D, Wehner TC, May B (1985) Electrophoretic variation and enzyme storage stability in cucumber. J Am Soc Hortic Sci 110:426–431Google Scholar
  41. Staub JE, Fredrick L, Marty TL (1987) Electrophoretic variation in cross-compatible wild diploid species of Cucumis. Can J Bot 65:792–798Google Scholar
  42. Tanksley SD, Orton TJ (1983) Isozymes in plant genetics and breeding, parts A & B. Elsevier, AmsterdamGoogle Scholar
  43. Weeden NF, Lamb RC (1987) Genetics and linkage analysis of 19 isozyme loci in apple. J Am Soc Hortic Sci 112:865–872Google Scholar
  44. Zamir D, Ladizinsky G (1983) Genetics of allozyme variants and linkage groups in lentil. Euphytica 33:329–336Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • L. D. Knerr
    • 1
  • J. E. Staub
    • 1
  • D. J. Holder
    • 1
  • B. P. May
    • 2
  1. 1.USDA/ARS, Department of HorticultureUniversity of WisconsinMadisonUSA
  2. 2.Department of Natural ResourcesCornell UniversityIthacaUSA

Personalised recommendations