Behavioral Ecology and Sociobiology

, Volume 18, Issue 5, pp 331–338

Prolonged mating in the milkweed leaf beetle Labidomera clivicollis clivicollis (Coleoptera: Chrysomelidae): a test of the “sperm-loading” hypothesis

  • Janis L. Dickinson


Female milkweed leaf beetles (Labidomera clivicollis clivicollis) frequently mate with more than one male, and pairs form mating associations which last for up to 42 h in the field. I tested the hypothesis that males remaining with females for long periods of time benefit by numerically overwhelming the sperm of their competitors. Male L.c.clivicollis copulated intermittently with females throughout an 11 hour period in the laboratory. When virgin females were allowed a single copulation, 94.3% of the sperm they received were located in the spermatheca immediately afterward. Males were not sperm-depleted, for they had large numbers of sperm available after one copulation (mean=230,000±43,200); the maximal number of sperm a male transferred to a female in 24 h was 30,500. There was a positive linear relationship between the number of sperm transferred and time up to 24 h after mounting (r2=0.178, P<0.003). These data suggest that males transfer increasing numbers of sperm throughout a 24-h-period. Mating duration was the most important determinant of paternity when females were placed with one male for 24 h and another male for 6 hours. Females whose first matings were longer showed first male sperm predominance (as determined by starch-gel electrophoresis), while females whose second matings were longer showed last male sperm predominance. In view of these data, it is puzzling that males do not inseminate with large numbers of sperm immediately after mounting the female. It is possible that female refractory behaviors make insemination difficult and favor prolonged mating by male milkweed leaf beetles.


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  1. Alcock JA (1976) Courtship and mating in Hippomelas planicosta (Coleoptera: Buprestidae). Coleopt Bull 30:343–348Google Scholar
  2. Altmann J (1984) Observational sampling methods for insect behavioral ecology. Fla Entomol 67:50–56CrossRefGoogle Scholar
  3. Eickwort KR (1977) Population dynamics of a relatively rare species of milkweed beetle (Labidomera clivicollis). Ecology 58:527–538CrossRefGoogle Scholar
  4. Eisner TE (1965) Defensive spray of a phasmid insect. Science 148:966–968PubMedCrossRefGoogle Scholar
  5. Gromko MH, Gilbert DG, Richmond RC (1984) Sperm transfer and use in the multiple mating system of Drosophila. In Smith RL (ed) Sperm competition and the evolution of animal mating systems. Academic Press, New York, pp 372–427Google Scholar
  6. Hagedorn HH, Turner S, Hagedorn EA, Pontecorvo D, Greenbaum P, Pfeiffer D, Wheelock G, Flanagan T (1977) Postemergence growth of the ovarian follicles of Aedes aegypti. J Insect Physiol 23:203–206PubMedCrossRefGoogle Scholar
  7. Hughes AL (1981) Differential male mating success in the white spotted sawyer Monochamus scutellatus (Coleoptera: Cerambycidae). Ann Entomol Soc Am 74:180–184CrossRefGoogle Scholar
  8. Johnson LK (1982) Sexual selection in a brentid weevil. Evolution 36:251–262CrossRefGoogle Scholar
  9. Johnson LK (1983) Reproductive behavior of Claeoderes bivittata (Coleoptera: Brentidae). Psyche 90:135–149CrossRefGoogle Scholar
  10. Kirkendall LR (1984) Long copulations and post-copulatory ‘escort’ behaviour in the locust leaf miner, Odontota dorsalis (Coleoptera: Chrysomelidae). J Nat Hist 18:905–919CrossRefGoogle Scholar
  11. Lanier DL, Estep DQ, Dewsbury DA (1979) Role of prolonged copulatory behavior in facilitating reproductive success in a competitive mating situation in laboratory rats. J Comp Physiol Psychol 93:781–792CrossRefGoogle Scholar
  12. Lindgren B (1968) Statistical theory. Macmillan New York, pp 241–252Google Scholar
  13. May B, Wright JE, Stoneking M (1979) Joint segregation of biochemical loci in Salmonidae: results from experiments with Salvelinus and review of the literature on other species. J Fisheries Res Board Can 36:1114–1128CrossRefGoogle Scholar
  14. McCauley DE, O'Donnell R (1984) The effect of multiple mating on genetic relatedness in larval aggregations of the imported willow leaf beetle (Plagiodera versicolora Coleoptera: Chrysomelidae). Behav Ecol Sociobiol 15:287–291CrossRefGoogle Scholar
  15. McVey ME, Smittle J (1984) Sperm precedence in the dragonfly Erythemis simplicicollis. J Insect Physiol 30:619–628CrossRefGoogle Scholar
  16. Oglesby JM, Lanier DL, Dewsbury DA (1981) The role of prolonged copulatory behavior in facilitating reproductive success in male Syrian golden hamsters (Mesocriceta auratus) in a competitive mating situation. Behav Ecol Sociobiol 8:47–54CrossRefGoogle Scholar
  17. Page RE Jr, Metcalf RA (1982) Multiple mating, sperm utilization, and social evolution. Am Nat 119:263–281CrossRefGoogle Scholar
  18. Parker GA (1970) Sperm competition and its evolutionary consequences in the insects. Biol Rev 45:525–567CrossRefGoogle Scholar
  19. Parker GA, Smith JL (1975) Sperm competition and the evolution of the precopulatory phase behavior in Locusta migratoria migratorioides. J Entomol [A] 49:155–171Google Scholar
  20. Schlager G (1960) Sperm precedence in the fertilization of eggs in Tribolium castaneum. Ann Entomol Soc Am 53:557–560CrossRefGoogle Scholar
  21. Silberglied RE, Shepherd JG, Dickinson JL (1984) Eunuchs: the role of apyrene sperm in Lepidoptera? Am Nat 123:255–265CrossRefGoogle Scholar
  22. Sillen-Tullberg B (1981) Prolonged copulation: a male ‘postcopulatory’ strategy in a promiscuous species, Lygaeus equestris (Heteroptera: Lygaeidae). Behav Ecol Sociobiol 9:283–289CrossRefGoogle Scholar
  23. Sivinski J (1983) Predation and sperm competition in the evolution of coupling durations in the stick insect Diaphomera veliei. In: Gwynne DT, Morris GK (eds) Orthopteran Mating Systems. Westview, Boulder, pp 147–162Google Scholar
  24. Smith RL (1979) Repeated copulation and sperm precedence: paternity assurance for a male brooding water bug. Science 205:1029–1031PubMedCrossRefGoogle Scholar
  25. Waage JK (1979) Adaptive significance of postcopulatory guarding of mates and nonmates by male Calopteryx maculata (Odonata). Behav Ecol Sociobiol 6:147–154CrossRefGoogle Scholar
  26. Waage JK (1984) Sperm competition and the evolution of Odonate mating systems. In: Smith RL (ed) Sperm competition and the evolution of animal mating systems. Academic Press, New York, pp 251–290Google Scholar
  27. Walker WF (1980) Sperm utilization strategies in nonsocial insects. Am Nat 115:780–799CrossRefGoogle Scholar
  28. Wilcox RS (1984) Male copulatory guarding enhances female foraging success in a water strider. Behav Ecol Sociobiol 15:171–174CrossRefGoogle Scholar
  29. Woodhead AP (1985) Sperm mixing in the cockroach Diploptera punctata. Evolution 39:159–164CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Janis L. Dickinson
    • 1
  1. 1.Department of EntomologyCornell UniversityIthacaUSA

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