Advertisement

Molecular and General Genetics MGG

, Volume 232, Issue 1, pp 7–11 | Cite as

Clustering of genes involved in nitrate assimilation in the cyanobacterium Synechococcus

  • Ignacio Luque
  • Antonia Herrero
  • Enrique Flores
  • Francisco Madueño
Article

Summary

A region of the genome of the cyanobacterium Synechococcus R2, that bears a cluster of genes involved in nitrate assimilation, has been cloned and the relative positions of some of the genes in the region have been determined. Mutations generated by insertion of an antibiotic-resistance gene cassette into the gene encoding nitrite reductase are associated with reduced expression of nitrate reductase; cotranscription of nitrate assimilation genes in the cluster is inferred from this finding.

Key words

Nitrate assimilation genes Synechococcus Cotranscription Genetic regulation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andriesse X, Bakker H, Weisbeek P (1990) Analysis of nitrate reduction genes in cyanobacteria. In: Ullrich WR, Rigano C, Fuggi A, Aparicio PJ (eds) Inorganic nitrogen in plants and microorganisms. Springer, Berlin, pp 303–307Google Scholar
  2. Ausubel FA, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) (1990) Current protocols in molecular biology. Greene Publishing and Wiley-Interscience, New YorkGoogle Scholar
  3. Buikema WJ, Haselkorn R (1991) Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 173:1879–1885Google Scholar
  4. Elhai JE, Wolk CP (1988) A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119–138Google Scholar
  5. Flores E, Ramos JL, Herrero A, Guerrero MG (1983) Nitrate assimilation by cyanobacteria. In: Papageorgiu GC, Packer L (eds) Photosynthetic prokaryotes: cell differentiation and function. Elsevier, New York, pp 363–387Google Scholar
  6. Golden SS, Sherman LA (1984) Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2. J Bacteriol 158:36–42Google Scholar
  7. Herrero A, Guerrero MG (1986) Regulation of nitrite reductase in the cyanobacterium Anacystis nidulans. J Gen Microbiol 132:2463–2468Google Scholar
  8. Herrero A, Flores E, Guerrero MG (1981) Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp strain 7119, and Nostoc sp strain 6719. J Bacteriol 145:175–180Google Scholar
  9. Kuhlemeier CJ, Logtenberg T, Stoorvogel W, Heugten HAA, Borrias WE, van Arkel GA (1984a) Cloning of nitrate reductase genes from the cyanobacterium Anacystis nidulans. J Bacteriol 159:36–41Google Scholar
  10. Kuhlemeier CJ, Teeuwsen WJP, Janssen MJT, van Arkel GA (1984b) Cloning of a thir nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle gene library. Gene 31:109–116Google Scholar
  11. Madueño F, Borrias WE, van Arkel GA, Guerrero MG (1988a) Isolation and characterization of Anacystis nidulans R2 mutants affected in nitrate assimilation: establishment of two new mutant types. Mol Gen Genet 213:223–228Google Scholar
  12. Madueño F, Vega-Palas MA, Flores E, Herrero A (1988b) A cytoplasmic-membrane protein repressible by ammonium in Synechococcus R2: altered expression in nitrate-assimilation mutants. FEBS Lett 239:289–291Google Scholar
  13. Meeks JC, Wolk CP, Lockau W, Schilling N, Shaffer PW, Chien W-S (1978) Pathways of assimilation of [13N]N2 and 13NH4 + by cyanobacteria with and without heterocysts. J Bacteriol 134:125–130Google Scholar
  14. Omata T (1991) Cloning and characterization of the nrtA gene that encodes a 45-kDa protein involved in nitrate transport in the cyanobacterium Synechocococcus PCC 7942. Plant Cell Physiol 32:151–157Google Scholar
  15. Omata T, Ohmori M, Arai N, Ogawa T (1989) Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate transport. Proc Natl Acad Sci USA 86:6612–6616Google Scholar
  16. van den Hondel CAMJJ, Verbeek S, van der Ende A, Weisbeek PJ, Borrias WE, van Arkel GA (1980) Introduction of transposon Tn901 into a plasmid of Anacystis nidulans: preparation for cloning in cyanobacteria. Proc Natl Acad Sci USA 77:1570–1574Google Scholar
  17. Vega-Palas MA, Madueño F, Herrero A, Flores E (1990) Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp strain PCC 7942. J Bacteriol 172:643–647Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Ignacio Luque
    • 1
  • Antonia Herrero
    • 1
  • Enrique Flores
    • 1
  • Francisco Madueño
    • 1
  1. 1.Instituto de Bioquimica Vegetal y Fotosintesis, Universidad de Sevilla y CSICFacultad de BiologíaSevillaSpain

Personalised recommendations