, Volume 30, Issue 5, pp 360–362 | Cite as

Opposite effects of diabetes and galactosaemia on adenosine triphosphatase activity in rat nervous tissue

  • J. E. Lambourne
  • D. R. Tomlinson
  • A. M. Brown
  • G. B. Willars
Rapid Communication


This study measured the ouabain-sensitive adenosine triphosphatase activity in sciatic nerve, lumbar dorsal root ganglia and superior cervical ganglia from control rats, rats with 8 weeks streptozotocin-induced diabetes and rats fed a diet containing 20% galactose for 8 weeks. Whilst the sciatic nerves of the diabetic rats showed a 42% reduction in ouabain-sensitive adenosine triphosphatase activity, the galactose-fed rats showed an increase of 124% (p<0.01 and p<0.005, respectively, compared to controls). There was also a reduction (by 30% compared to controls; p<0.05) in the ouabain-sensitive adenosine triphosphatase activity of the dorsal root ganglia from the diabetic rats, but their superior cervical ganglia did not show a significant fall. The ganglia of the galactosaemic rats showed no change in ouabain-sensitive adenosine triphosphatase activity compared to controls. These changes coexisted with increases in appropriate polyol pathway metabolites in all tissues of both diabetic and galactosaemic rats. There were also depletions of myo-inositol in the sciatic nerves and dorsal root ganglia of diabetic and galactosaemic rats, but their superior cervical ganglia contained levels of myo-inositol which were similar to those of controls. The nerves of the galactosaemic rats showed increased water content; the nerves of the diabetic rats did not. The data argue against a simple relationship between myoinositol depletion and impaired Na/K adenosine triphosphatase activity in association with exaggerated polyol pathway flux in peripheral nervous tissue.

Key words

adenosine triphosphatase diabetic neuropathies galactosaemia myo-inositol polyol pathway streptozotocindiabetes 


  1. 1.
    Das PK, Bray GM, Aguayo AJ, Raminsky M (1976) Diminished ouabain-sensitive, sodium-potassium ATPase activity in sciatic nerves of rats with streptozotocin-induced diabetes. Exp Neurol 53: 285–288Google Scholar
  2. 2.
    Greene DA, Lattimer SA (1983) Impaired rat sciatic nerve sodium-potassium ATPase in acute streptozotocin diabetes and its correction by dietary myo-inositol supplementation. J Clin Invest 72: 1058–1063Google Scholar
  3. 3.
    Green RJ, King RHM, Thomas PK, Baron DN (1985) Sodium-potassium-ATPase activity in the dorsal root ganglia of rats with streptozotocin-induced diabetes. Diabetologia 28: 104–107Google Scholar
  4. 4.
    Greene DA, Mackway AM (1986) Decreased myo-inositol content and Nas+-Ks+-ATPase activity in the superior cervical ganglion of STZ-diabetic rat and prevention by aldose reductase inhibition. Diabetes 35: 1106–1108Google Scholar
  5. 5.
    Greene DA, Lattimer SA (1984) Action of sorbinil in diabetic peripheral nerve: relationship of polyol (sorbitol) pathway inhibition to a myo-inositol-mediated defect in sodium-potassium ATPase activity. Diabetes 33: 712–716Google Scholar
  6. 6.
    Stewart MA, Sherman WR, Kurien MM, Moonsammy GI, Wisgerhof M (1967) Polyol accumulations in nervous tissue of rats with experimental diabetes and galactosemia. J Neurochem 14: 1057–1066Google Scholar
  7. 7.
    Kador PF, Kinoshita JH (1985) Role of aldose reductase in the development of diabetes associated complications. Am Med J 79: 8–12Google Scholar
  8. 8.
    Greene DA, Lattimer S, Ulbrecht J, Carroll P (1984) Glucose-induced alterations in nerve metabolism: current perspective on the pathogenesis of diabetic neuropathy and future directions for research and therapy. Diabetes Care 8: 290–299Google Scholar
  9. 9.
    Jaworek D, Gruber W, Bergmeyer HU (1974) Adenosine-5′-diphosphate and adenosine-5′-monophosphate. In Bergmeyer HU (ed) Methods of enzymatic analysis, Vol 4, 2nd English edn, Verlag Chemie GmbH/Academic Press: Weinheim/New York, London, pp 2127–2129Google Scholar
  10. 10.
    Mayer JH, Tomlinson DR (1983) Prevention of defects of axonal transport and nerve conduction velocity by oral administration of myo-inositol or an aldose reductase inhibitor in streptozotocindiabetic rats. Diabetologia 25: 433–438Google Scholar
  11. 11.
    Mizisin AP, Powell HC, Myers RR (1986) Edema and increased endoneurial sodium in galactose neuropathy. J Neurol Sci 74: 35–43Google Scholar
  12. 12.
    Llewelyn JG, Simpson CMF, Thomas PK, King RHM, Hawthorne JN (1986) Changes in sorbitol, myo-inositol and lipid inositol in dorsal root and sympathetic ganglia from streptozotocin-diabetic rats. Diabetologia 29: 876–881Google Scholar
  13. 13.
    Gabbay KH (1973) Role of sorbitol pathway in neuropathy. Adv Metab Disord 2 [Suppl 2]: 417–424Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • J. E. Lambourne
    • 1
  • D. R. Tomlinson
    • 1
  • A. M. Brown
    • 1
  • G. B. Willars
    • 1
  1. 1.Department of Physiology and PharmacologyMedical School, Queen's Medical CentreNottinghamUK

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