Zeitschrift für vergleichende Physiologie

, Volume 53, Issue 4, pp 449–474 | Cite as

Eine Analyse des Melanophorenstoffwechsels Untersuchungen an Rana temporaria

  • Manfred Fuchs
Article
Received:

Summary

  1. 1.

    The oxygen consumption of the melanophores in isolated pieces of skin from Rana temporaria is measured in a Warburg apparatus.

     
  2. 2.

    The metabolic rate of skin from different individuals varies considerably. However, several skin samples of the same animal show synchronized metabolic rates. Therefore it is possible to use skin pieces from the same animal for experiment and control.

     
  3. 3.

    Pigment dispersion by MDH or ACTH increases the oxygen consumption from skin of the back which is rich in melanophores (100 000–130 000 melanophores/5 cm2 skin). The metabolic rate of skin from the ventral side with less than 10 000 melanophores/5 cm2 is not effected measurably by the dispersing agents used.

     
  4. 4.

    The metabolic increase is correlated significantly with the number of melanophores reacting to the dispersing substances. As shown by a regression analysis the oxygen consumption increases linearly with the number of responding melanophores, namely one percent per 10 000 melanophores.

     
  5. 5.

    Pigment dispersion caused by coffein increases the metabolic rate of the melanophores similarly as the action of MDH and ACTH. Accordingly, the metabolic increase is not bound specifically to these hormones, but only to pigment dispersion.

     
  6. 6.

    There is no decrease in oxygen consumption after maximum dispersion is reached. Consequently, an increased metabolic rate is required to maintain the state of dispersion.

     
  7. 7.

    No oxygen is necessary for pigment aggregation. The metabolic rate caused by pigment dispersion decreases to the basic metabolism of the tissue.

     
  8. 8.

    The speed of pigment movement is correlated with the change of melanophore oxygen consumption.

     
  9. 9.

    The effect of ACTH is prevented by blocking cytochromoxydase with KCN. The oxygen which is necessary for pigment dispersion enters into the respiratory chain. Therefore, energy from oxydative metabolic processes is required for pigment dispersion as well as for the maintainance of the state of dispersion.

     
  10. 10.

    The process of pigment dispersion and the maintainance of the state of dispersion are the active phases of the melanophores.

     
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Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • Manfred Fuchs
    • 1
  1. 1.Zoologisches Institut der Universität Frankfurt a. M.Deutschland

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