Calcified Tissue International

, Volume 55, Issue 2, pp 109–113 | Cite as

Regulation of lymphocyte calcitonin receptors by interleukin-1 and interleukin-6

  • J. J. Body
  • G. Fernandez
  • M. Lacroix
  • P. Vandenbussche
  • J. Content
Laboratory Investigations
Received:
Accepted:

Abstract

We have reported the existence of specific and high-affinity calcitonin (CT) receptors on normal human T-lymphocytes. Because of the increasingly recognized importance of interleukin-1 (IL-1) and IL-6 in the control of bone metabolism, we have examined their influence on the binding parameters of labeled salmon calcitonin (sCT) on lymphocytes. After a 24-hour incubation, IL-1 at 100–5000 U/ml and IL-6, at 1–1000 U/ml, decreased the apparent number of CT binding sites (Bmax) on T-lymphocytes. The effects of IL-6 on purified T-lymphocytes were dose related and 100 U of IL-6/ml reduced sCT binding to 57±16% (mean ± SD) of the control values (n=6). There was no significant change in CT binding affinity (Kd, 0.71±0.54x10-10 M for controls versus 0.90±0.55x10-10 M after IL-6) and the decrease in Bmax was reversible after 48 hours. The effects of IL-1 appeared to be mediated through an increased production of IL-6 as they were neutralized by a polyclonal antiserum against IL-6. Added alone, the antiserum caused a slight increase in the apparent number of CT binding sites on T-lymphocytes to 115±5% of control values (n=3). In summary, IL-1 and IL-6 can induce a marked apparent loss of CT binding sites on normal T-lymphocytes at concentrations known to be active on bone metabolism. The contributions of our observations to the osteolytic activity of these cytokines deserve further investigation.

Key words

Calcitonin Interleukin-6 Cytokine Bone resorption Lymphocyte 
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Copyright information

© Springer-Verlag New York Inc 1994

Authors and Affiliations

  • J. J. Body
    • 1
    • 2
  • G. Fernandez
    • 1
    • 2
  • M. Lacroix
    • 1
    • 2
  • P. Vandenbussche
    • 3
  • J. Content
    • 3
  1. 1.Bone Metabolism Unit, Service de Médecine, Institut J. BordetUniversité Libre de BruxellesBrusselsBelgium
  2. 2.Laboratoire d'Investigation Clinique H.J. Tagnon, Institut J. BordetUniversité Libre de BruxellesBrusselsBelgium
  3. 3.Institut Pasteur du BrabantBrusselsBelgium

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