Urological Research

, Volume 24, Issue 1, pp 55–60 | Cite as

Enhanced gene expression of transforming growth factor-α and c-met in rat urinary bladder cancer

  • M. Inui
  • N. Nishi
  • A. Yasumoto
  • I. Takenaka
  • H. Miyanaka
  • K. Matsumoto
  • T. Nakamura
  • F. Wada
Original Paper


To investigate the roles of growth factors in bladder cancer, changes in the expression of messenger RNAs (mRNAs) for several growth factors and their receptors were examined during rat bladder carcinogenesis induced with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN). Northern blot analysis showed that the contents of mRNAs for transforming growth factor-α (TGF-α) and c-met/hepatocyte growth factor (HGF) receptor increased with BBN treatment. Epidermal growth factor (EGF) receptor mRNA was hardly affected by the treatment; while mRNA for fibroblast growth factor (FGF) receptor 1 and transforming growth factor-β (TGF-β) type II receptor decreased with BBN treatment. A rat bladder tumor cell line, NBT-II, expressed both TGF-α and c-met mRNAs, and HGF showed apparent scattering and growth-stimulating effects on the cells. These results indicate the possibility that TGF-α produced by a bladder cancer, in addition to urinary EGF, plays a role in the development of bladder cancer, and that enhanced cell motility due to activation of the c-met/HGF receptor participates in the invasion and metastasis of the cancer cells.

Key words

Bladder carcinoma Gene expression Growth factors Growth factor receptors Cell motility 


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Inui
    • 1
  • N. Nishi
    • 2
  • A. Yasumoto
    • 1
  • I. Takenaka
    • 1
  • H. Miyanaka
    • 3
  • K. Matsumoto
    • 4
  • T. Nakamura
    • 4
  • F. Wada
    • 2
  1. 1.Department of UrologyKagawa Medical SchoolKagawaJapan
  2. 2.Department of EndocrinologyKagawa Medical SchoolKagawaJapan
  3. 3.Research Equipment CenterKagawa, Medical SchoolKagawaJapan
  4. 4.Biomedical Research CenterOsaka University School of MedicineSuita, OsakaJapan

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