Applied Microbiology and Biotechnology

, Volume 30, Issue 4, pp 375–380 | Cite as

DNA mediated transformation of the filamentous fungus Curvularia lunata using a dominant selectable marker

  • H. D. Osiewacz
  • A. Weber
Applied Genetics and Regulation


A DNA mediated transformation system was developed for the filamentous fungus Curvularia lunata. Incubation of protoplasts with a plasmid (pAN7-1) which contains the Escherichia coli hygromycin B phosphotransferase gene (hph) fused to an Aspergillus nidulans promoter resulted in the formation of hygromycin B resistant transformants. DNA-DNA hybridization experiments revealed that plasmid pAN7-1 became integrated into the fungal genome. All transformants analyzed so far proved to be stable even after vegetative growth for three months without selective pressure.


Escherichia Coli Aspergillus Selective Pressure Vegetative Growth Hygromycin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • H. D. Osiewacz
    • 1
  • A. Weber
    • 2
  1. 1.Lehrstuhl für Allgemeine BotanikRuhr-UniversitätBochumFederal Republic of Germany
  2. 2.Schering AGMikrobiologische EntwicklungBerlin-Charlottenburg

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