Current Microbiology

, Volume 30, Issue 3, pp 149–153 | Cite as

Detection of mip-like sequences and mip-related proteins within the family Rickettsiaceae

  • Nicholas P. Cianciotto
  • William O'Connell
  • Gregory A. Dasch
  • Louis P. Mallavia


The Mip surface protein, a prokaryotic analog of the FK506-binding proteins, enhances the ability of Legionella pneumophila to infect macrophages and protozoa. Using mip-specific probes and low-stringency Southern hybridizations, we have detected DNA sequences homologous to mip within Coxiella burnetii and Rochalimaea quintana. Using specific anti-Mip antisera and immunoblot analysis, we also detected Mip-related proteins within these bacteria as well as within Rickettsia and Ehrlichia species. These data suggest that Mip-related proteins have broad significance for host-parasite interactions. However, they also indicate that care must be exercised when using mip probes or anti-Mip antibodies for the detection of Legionella organisms in water or clinical samples.


Clinical Sample Surface Protein Immunoblot Analysis Southern Hybridization Broad Significance 
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Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Nicholas P. Cianciotto
    • 1
  • William O'Connell
    • 1
  • Gregory A. Dasch
    • 2
  • Louis P. Mallavia
    • 3
  1. 1.Department of Microbiology-ImmunologyNorthwestern UniversityChicagoUSA
  2. 2.Viral and Rickettsial Diseases Program, Naval Medical Research InstituteNational Naval Medical CenterBethesdaUSA
  3. 3.Department of MicrobiologyWashington State UniversityPullmanUSA

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