Acta Neuropathologica

, Volume 87, Issue 2, pp 155–160

Expression of αB-crystallin in Alzheimer's disease

  • K. Renkawek
  • C. E. M. Voorter
  • G. J. C. G. M. Bosman
  • F. P. A. van Workum
  • W. W. de Jong
Regular Paper

DOI: 10.1007/BF00296185

Cite this article as:
Renkawek, K., Voorter, C.E.M., Bosman, G.J.C.G.M. et al. Acta Neuropathol (1994) 87: 155. doi:10.1007/BF00296185
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Abstract

αB-crystallin is a member of the small heatshock protein family. Under pathological conditions, the expression of αB-crystallin increases in proliferating astrocytes, which suggests that this protein, in addition to glial fibrillary acidic protein (GFAP), can be a marker for gliosis in neurodegenerative diseases. Immunoblotting and immunohistochemical methods were used for the detection of αB-crystallin in the brains of Alzheimer's disease (AD) patients and nondemented controls. An increase in αB-cyrstallin expression was found in the brains of AD patients. Immunoreaction was present in reactive astrocytes, microglia, and oligodendrocytes, indicating that all types of glia respond to the stress associated with AD pathology. Colocalization of GFAP and αB-crystallin was found in fibrous astrocytes. However, the intensity and range of αB-crystallin expression appeared to be limited as compared with the large increase in the number of GFAP-positive astrocytes. This indicates that expression of αB-crystallin is not a marker for gliosis in AD. Immunoreactivity to αB-crystallin in both astrocytes and microglia was found mainly restricted to areas with senile plaques and neurofibrillary tangles, suggesting the association of αB-crystallin with amyloid deposition in AD.

Key words

αB-crystallin Heat-shock proteins Reactive glia Alzheimer's disease 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • K. Renkawek
    • 1
    • 2
  • C. E. M. Voorter
    • 1
    • 2
  • G. J. C. G. M. Bosman
    • 1
    • 2
  • F. P. A. van Workum
    • 1
    • 2
  • W. W. de Jong
    • 1
    • 2
  1. 1.Institute of Neurology, Research Laboratory of Morphological NeurologyUniversity of NijmegenNijmegenThe Netherlands
  2. 2.Department of BiochemistryUniversity of NijmegenNijmegenThe Netherlands

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