Human Genetics

, Volume 35, Issue 1, pp 83–89 | Cite as

Laser UV microirradiation of interphase nuclei and post-treatment with caffeine

A new approach to establish the arrangement of interphase chromosomes
  • Christian Zorn
  • Thomas Cremer
  • Christoph Cremer
  • Jürgen Zimmer
Original Investigations


Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three categories: I) intact metaphase figures, II) chromosome damage confined to a small area of the metaphase spread, III) mitotic figures with damage on all chromosomes. Category III might be the consequence of a non-localized distortion of nuclear metabolism. By contrast, category II may reflect localized DNA damage induced by microirradiation, which could not be efficiently repaired due to the effect of caffeine. If this interpretation is right, in metaphase figures of category II chromosome damage should occur only at the irradiation site. The effect might then be used to investigate neighbourhood relationships of individual chromosomes in the interphase nucleus.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Barr, H. J., Ellison, J. R.: Ectopic pairing of chromosome regions containing chemically similar DNA. Chromosoma (Berl.) 39, 53–61 (1972)Google Scholar
  2. Comings, D. E.: The rationale of an ordered arrangement of chromatin in the interphase nucleus. Amer. J. hum. Genet. 20, 440–460 (1968)PubMedGoogle Scholar
  3. Comings, D. E.: Implications of somatic recombination and sister chromatid exchange in Bloom's syndrome and cells treated with mitomycin C. Humangenetik 28, 191–196 (1975)PubMedGoogle Scholar
  4. Cremer, C., Cremer, T., Zorn, C., Schoeller, L.: Effect of laser uv-microirradiation (λ=2573 Å) on proliferation of Chinese hamster cells. Radiat. Res. 66, 106–121 (1976)PubMedGoogle Scholar
  5. Cremer, C., Zorn, C., Cremer, T.: An ultraviolet laser microbeam for 257 nm. Microsc. Acta 75, 331–337 (1974)PubMedGoogle Scholar
  6. Deaven, L. L., Petersen, D. F.: The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses. Chromosoma (Berl.) 41, 129–144 (1973)CrossRefGoogle Scholar
  7. Hens, L., Kirsch-Volders, M., Susanne, C., Galperin-Lemaître, H.: Relative positions of trypsin banded homologous chromosomes in human (♀) metaphase figures. Humangenetik 28, 303–311 (1975)PubMedGoogle Scholar
  8. Kihlman, B. A.: Effects of caffeine on the genetic material. Mutat. Res. 26, 53–71 (1974)PubMedGoogle Scholar
  9. Kihlman, B. A., Sturelid, S., Hartley-Asp, B., Nilsson, K.: The enhancement by caffeine of the frequencies of chromosomal aberrations induced in plant and animal cells by chemical and physical agents. Mutat. Res. 26, 105–122 (1974)PubMedGoogle Scholar
  10. Moreno, G., Salet, C.: Unscheduled DNA-synthesis after ultraviolet microirradiation of the cell nucleus. Radiat. Res. 58, 52–59 (1974)PubMedGoogle Scholar
  11. Nilsson, K., Lehmann, A. R.: The effect of methylated oxypurines on the size of newlysynthesized DNA and on the production of chromosome aberrations after uv-irradiation of Chinese hamster cells. Mutat. Res. 30, 255–266 (1975)PubMedGoogle Scholar
  12. Nur, U.: Random arrangement of chromosomes in a radial metaphase configuration. Chromosoma (Berl.) 40, 263–267 (1973)Google Scholar
  13. Sakharov, V. N., Voronkova, L. N., Pyruzyan, L. A., Lomakina, L. Y.: Partial chromosomal labelling induced by ultraviolet microbeam irradiation of mitotic cells. Nature (Lond.) 260, 784–785 (1976)Google Scholar
  14. Schmid, M., Vogel, W., Krone, W.: Attraction between centric heterochromatin of human chromosomes. Cytogenet. Cell Genet. 15, 66–80 (1975)PubMedGoogle Scholar
  15. Schor, S. L., Johnson, R. T., Waldren, C. A.: Changes in the organization of chromosomes during the cell cycle: Response to ultraviolet light. J. Cell Sci. 17, 539–565 (1975)PubMedGoogle Scholar
  16. Schwarzacher, H. G.: Chromosomes in mitosis and interphase. Berlin-Heidelberg-New York: Springer 1976Google Scholar
  17. Singh, R. J., Röbbelen, G., Okamoto, M.: Somatic association at interphase studied by Giemsa banding technique. Chromosoma (Berl.) 56, 265–273 (1976)Google Scholar
  18. Sperling, K., Rao, P. N.: The phenomenon of premature chromosome condensation: Its relevance to basic and applied research. Humangenetik 23, 235–258 (1974)PubMedGoogle Scholar
  19. Vogel, F., Schroeder, T. M.: The internal order of the interphase nucleus. Humangenetik 25, 265–297 (1974)PubMedGoogle Scholar
  20. Warburton, D. Naylor, A. F., Warburton, F. E.: Spatial relations of human chromosomes identified by Quinacrine fluorescence at metaphase. Humangenetik 18, 297–306 (1973)PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Christian Zorn
    • 1
  • Thomas Cremer
    • 1
  • Christoph Cremer
    • 1
  • Jürgen Zimmer
    • 1
  1. 1.Institut für Humangenetik und Anthropologie der UniversitätFreiburg i. Br.Federal Republic of Germany

Personalised recommendations