Human Genetics

, Volume 35, Issue 1, pp 27–34 | Cite as

Chromosomal constitution of nucleolus-associated chromatin in man

  • A. Stahl
  • M. Hartung
  • A. M. Vagner-Capodano
  • C. Fouet
Original Investigations

Summary

Use of specific stains permits analysis of the frequency of nucleolus-associated heterochromatin in chromosomes 1 and 9 from human fibroblasts. In 81% of interphase nuclei the heterochromatic segment of both No. 1 chromosomes is associated with the nucleolus, while in 19% only one heterochromatic segment shows such an association with the other occupying a random position in the nucleoplasm. The nucleolar association of chromosome 9 heterochromatin is less constant: in 42.3% of the nuclei both segments are associated with the nucleolus, in 39% of the nuclei only one heterochromatic segment presents such an association, and in 18.7% neither of the two heterochromatic segments is in nucleolar association. In 6% of the cells, one or two chromosome 9 heterochromatic segments are in contact with the nuclear membrane.

In situ hybridization using tritium-labeled 28S and 18S RNA shows that in the interphase nucleus the acrocentric short arms, carriers of ribosomal cistrons, are associated with the nucleolus.

These observations demonstrate the complexity of the nucleolus-associated chromatin which, in addition to segments of chromosomes 1, 9, 13, 14, 15, 21 and 22, may include the Y chromosome. They also confirm that the nucleolus constitutes one of the orientation points determining the relative localization of chromosomes in the interphase nucleus.

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References

  1. Bernhard, W., Granboulan, N.: The fine structure of the cancer cell nucleus. Expt. Cell Res. suppl. 9, 19–53 (1963)Google Scholar
  2. Bobrow, M., Pearson, P. L., Gollacott, H. E. A. C.: Para-nucleolar position of the human Y chromosome in interphase nuclei. Nature (Lond.) 232, 556–557 (1971)Google Scholar
  3. Bourgeois, C. A.: Distribution of mitomycin C-induced damage in human chromosomes with special reference to regions of repetitive DNA. Chromosoma 48, 203–211 (1974)CrossRefPubMedGoogle Scholar
  4. Caspersson, T.: Cell growth and cell function. A cytochemical study. New York: Norton and Company 1950Google Scholar
  5. Ferguson-Smith, M. A.: The sites of nucleolus formation in human pachytene chromosomes. Cytogenetics 3, 124 (1964)Google Scholar
  6. Gagne, R.: Association entre le corpuscle fluorescent interphasique correspondant au chromosome Y de l'homme et le nucléole. Exc. Med. Intern. Congr. Ser. 233;1, 70 (1971)Google Scholar
  7. Gagne, R., Laberge, C.: Specific cytological recognition of the heterochromatic segment of number 9 chromosome in man. Exp. Cell Res. 73, 239–242 (1972)PubMedGoogle Scholar
  8. Gagne, R., Laberge, C., Tanguay, R.: Aspect cytologique et localisation intranucléaire de l'hétérochromatine constitutive des chromosomes C9 chez l'Homme. Chromosoma (Berl.) 41, 159–166 (1973)CrossRefGoogle Scholar
  9. Gall, J. G., Pardue, M. L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. nat. Acad. Sci. (USA) 63, 378–383 (1969)Google Scholar
  10. Goessens, G.: Les centres fibrillaires des nucléoles des cellules tumorales d'Ehrlich. C. R. Acad. Sc. 277, 325–327 (1973)Google Scholar
  11. Goessens, G., Lepoint, A.: The fine structure of the nucleolus during interphase and mitosis in Ehrlich tumour cells cultivated in vitro. Expt. Cell. Res. 87, 62–72 (1974)Google Scholar
  12. Goessens, G.: Etude ultrastructurale des nucléoles au cours du cycle cellulaire. Thèse de Doctorat. Université de Liège (1975)Google Scholar
  13. Gosden, J. R., Mitchell, A. R., Buckland, R. A., Clayton, R. P., Evans, H. J.: The location of four human satellite DNAs on human chromosomes. Expt. Cell Res. 92, 148–158 (1975)Google Scholar
  14. Hartung, M., Fouet, C., Stahl, A.: L'association au nucléole de l'hétérochromatine juxta-centromérique du chromosome 1 de l'Homme. Ann. Génét. 18, no. 4, 247–249 (1975)Google Scholar
  15. Henderson, A. S., Warburton, D., Atwood, K. C.: Location of ribosomal RNA in the human chromosome complement (rRNA satellite regions acrocentric chromosomes). Proc. nat. Acad. Sci. (USA) 69, 3394–3398 (1972)Google Scholar
  16. Jones, K. W., Corneo, G.: Location of satellite and homogenous DNA sequences on human chromosomes. Nature New Biology 233, 268–271 (1971)PubMedGoogle Scholar
  17. Jones, K. W., Corneo, G., Ginelli, E., Bobrow, M.: The chromosomal location of human satellite DNA III. Chromosoma 42, 445–451 (1973)PubMedGoogle Scholar
  18. Morad, M., Jonasson, J., Lindsten, J.: Distribution of mitomycin C induced breaks on human chromosomes. Hereditas 74, 273–282 (1973)PubMedGoogle Scholar
  19. Ohno, S., Trujillo, J. M., Kaplan, W. D., Kinosita, R.: Nucleolus-organizer in the causation of chromosomal anomalies in man. Lancet 1961 II, 123Google Scholar
  20. Pardo, D., Luciani, J. M., Stahl, A.: Localisation par hybridation in situ des gènes des ARN 28S et 18S dans les chromosomes somatiques humains. Ann. Génét. 18, 105–109 (1975)Google Scholar
  21. Saunders, G. F., Hsu, T. C., Getz, M. J., Simes, E. L., Arrighi, F. E.: Location of human satellite DNA in human chromosomes. Nature New Biology 236, 244–246 (1972)Google Scholar
  22. Smetana, K., Busch, H.: Studies on the ultrastructure of the nucleoli of the Walker tumor and rat liver. Cancer Res. 24, 537–557 (1964)PubMedGoogle Scholar
  23. Schmid, M., Vogel, W., Krone, W.: Attraction between centric heterochromatin of human chromosomes. Cytogenet. Cell Genet. 15, 66–80 (1975)PubMedGoogle Scholar
  24. Stahl, A., Luciani, J. M., Devictor, M., Capodano, A. M., Gagne, R.: Constitutive heterochromatin and micronucleoli in the human oocyte at diplotene stage. Humangenetik 26, 315–327 (1975)PubMedGoogle Scholar
  25. Steffensen, D. M., Duffey, P.: Localisation of 5S ribosomal RNA genes on human chromosome 1. Nature 252, 741–743 (1974)PubMedGoogle Scholar
  26. Steffensen, D. M., Prensky, W., Dufy, P.: Localization of the 5S ribosomal RNA genes in the human genome. In: New Haven Conference (1973). First international workshop on human gene mapping, pp. 153–154. Birth defects: Original Articles Series, Vol. X (The National Foundation, New York 1974)Google Scholar
  27. Therkelsen, A. J., Petersen, G. B.: Frequency of “Y” chromatin body in human skin fibroblasts in tissue culture, and its relation to growth phase. Expt. Cell Res. 65, 473–475 (1971)Google Scholar
  28. Vogel, F., Schroeder, T. M.: The internal order of the interphase nucleus. Humangenetik 25, 265–297 (1974)PubMedGoogle Scholar
  29. Wyandt, H. E., Iorio, R. J.: Human Y chromatin. 3. The nucleolus. Expt. Cell Res. 81, 468–473 (1973)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • A. Stahl
    • 1
  • M. Hartung
    • 1
  • A. M. Vagner-Capodano
    • 1
  • C. Fouet
    • 1
  1. 1.Laboratoire d'Histologie et Embryologie IIFaculté de MédecineMarseile Cedex 4France

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