An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures
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By using mercury(II)-chloride (HgCl2) and Dl-6-fluorotryptophan (6FT) as positive selection conditions we were able to show that N-methyl-N′-nitro-N-nitrosoguanidine (NG) is an effective mutagen for Petunia hybrida suspension cells.
A number of the 205 calli resistant to HgCl2 and 17 calli resistant to 6FT were isolated. The highest mutation frequency was 1.0 x 10−5 and 2.0 x 10−6 for HgCl2 and 6FT, respectively. A preliminary characterization of the mutants is presented.
A significant increase in the number of drug-resistant calli was only obtained at NG-concentrations (5–40 μg/ml) that had no observable effect on the survival of the mutagenized cultures.
Key wordsMutagenesis Petunia hybrida cell lines Drug resistant mutants
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