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Theoretical and Applied Genetics

, Volume 55, Issue 3–4, pp 101–106 | Cite as

An effective chemical mutagenesis procedure for Petunia hybrida cell suspension cultures

  • C. M. Colijn
  • A. J. Kool
  • H. J. J. Nijkamp
Article

Summary

By using mercury(II)-chloride (HgCl2) and Dl-6-fluorotryptophan (6FT) as positive selection conditions we were able to show that N-methyl-N′-nitro-N-nitrosoguanidine (NG) is an effective mutagen for Petunia hybrida suspension cells.

A number of the 205 calli resistant to HgCl2 and 17 calli resistant to 6FT were isolated. The highest mutation frequency was 1.0 x 10−5 and 2.0 x 10−6 for HgCl2 and 6FT, respectively. A preliminary characterization of the mutants is presented.

A significant increase in the number of drug-resistant calli was only obtained at NG-concentrations (5–40 μg/ml) that had no observable effect on the survival of the mutagenized cultures.

Key words

Mutagenesis Petunia hybrida cell lines Drug resistant mutants 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • C. M. Colijn
    • 1
  • A. J. Kool
    • 1
  • H. J. J. Nijkamp
    • 1
  1. 1.Department of Genetics, Biological LaboratoryFree UniversityAmsterdamthe Netherlands

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