Urological Research

, Volume 21, Issue 1, pp 49–53 | Cite as

A method for isolating smooth muscle cells from pig urinary bladder with low concentrations of collagenase and papain: the relation between calcium concentration and isolated cell length

  • R. Schot
  • E. van Asselt
  • R. van Mastrigt
Original Articles


The present study describes a method for isolating single smooth muscle cells from pig urinary bladder using a continuous resuspension device. Low concentrations of collagenase and papain were sufficient to obtain a high yield of viable smooth muscle cells, which remained viable for about 3–4 h as tested with fluorescein diacetate. Addition of fetal calf serum increased the lifespan of the isolated cells and the percentage of contractile smooth muscle cells, but caused spontaneous shortening of the cells. The length and volume of the isolated smooth muscle cells depended on the calcium concentration used in the isolation buffer solution. The isolated muscle cells were apparently relaxed if a calcium concentration less than 1.0 mmol/l was used in the isolation medium. In higher calcium concentrations the isolated cells were significantly shorter, probably as a result of a contraction caused by mechanical stimulation of the cells during the isolation procedure.

Key words

Bladder Calcium Cell isolation Contration Smooth muscle 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • R. Schot
    • 1
  • E. van Asselt
    • 1
  • R. van Mastrigt
    • 1
  1. 1.Department of UrologyErasmus University RotterdamRotterdamThe Netherlands

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