Applied Microbiology and Biotechnology

, Volume 21, Issue 3–4, pp 143–147 | Cite as

Effects of magnesium ion and chelating agents on enzymatic production of ATP from adenine

  • Tatsuro Fujio
  • Akira Furuya
Biotechnology

Summary

As reported previously, enzymatic production of ATP from adenine by resting cells of Brevibacterium ammoniagenes (Fujio and Furuya 1983) accumulated 13.0 mg of ATP · Na2 · 3H2O/ml, but ATP formation ceased within 6–8 h. Simultaneous addition of magnesium ion and phytic acid, a chelator of divalent cations, allowed ATP formation to continue longer, and 24.2 mg of ATP · Na2 · 3H2O/ml was accumulated in 10 h. However, ATP formation ceased thereafter.

This second cessation was found to be caused by the lack of magnesium ion active as a co-factor (Mgact). The Mgact was tentatively taken as the difference between soluble magnesium ion (Mgsol) and the ion chelated by an equimolar amount of ATP (MgATP), namely Mgact=Mgsol-MgATP. In order to provide Mgact, sufficient phytic acid had to be added at the beginning of the reaction and magnesium ion was also added intermittently. Under these conditions ATP formation continued further, and the rate of ATP formation was increased; 37.0 mg of ATP · Na2 · 3H2O/ml was accumulated in 13 h.

Since whole culture broth is preferable to frozen cells as a practical enzyme source, the conditions neccessary for use of whole culture broth of B. ammoniagenes were also investigated.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Asada M, Nakanishi K, Matsuno R, Kariya Y, Kimura A, Kamikubo T (1978) Continuous ATP regeneration utilizing glycolysis and kinase systems of yeast. Agric Biol Chem 42:1533–1538Google Scholar
  2. Fujio T, Furuya A (1983) Production of ATP from adenine by Brevibacterium ammoniagenes. J Ferment Technol 61:261–267Google Scholar
  3. Fujio T, Kotani Y, Furuya A (1984) Production of 5′-guanylic acid by enzymatic conversion of 5′-xanthylic acid. J Ferment Technol 62:131–137Google Scholar
  4. Murata K, Tani K, Chibata I (1981) Glycolytic pathway as an ATP regeneration system and its application to the production of glutathione and NADP. Enzyme Microbiol Technol 3:233–242Google Scholar
  5. Nara T, Misawa M, Kinoshita S (1968) Pantothenate, thiamine and manganese in 5′-purine ribonucleotide production by Brevibacterium ammoniagenes. Agric Biol Chem 32:1153–1161Google Scholar
  6. Shimizu S, Tani Y, Ogata K (1979) Synthesis of coenzyme A and its biosynthetic intermediates by microbial processes. In: McCormick DB, Wright LD (eds) Methods in enzymol, vol 62. Academic Press, New York, pp 236–245Google Scholar
  7. Shimosaka M, Fukuda Y, Kimura A (1981) Application of plasmid to ATP production by E. coli. Agric Biol Chem 45:1025–1027Google Scholar
  8. Shimosaka M, Fukuda Y, Murata K, Kimura A (1982) Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli. J Bacteriol 152:98–103Google Scholar
  9. Tachiki T, Matsumoto H, Yano T, Tochikura T (1981) Glutamine production by coupling with energy transfer employing with yeast cell-free extracts and Gluconobacter glutamine synthetase. Agric Biol Chem 45:705–710Google Scholar
  10. Tanaka H, Sato Z, Nakayama K, Kinoshita S (1968) Formation of ATP, GTP and their related substances by Brevibacterium ammoniagenes. Agric Biol Chem 32:721–726Google Scholar
  11. Tochikura T, Kuwahara M, Yagi S, Okamoto H, Tominaga T, Kato T, Ogata K (1967) Fermentation and metabolism of nucleic acid-related compounds in yeasts. J Ferment Technol 45:511–529Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Tatsuro Fujio
    • 1
  • Akira Furuya
    • 1
  1. 1.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co.Machida-shi, TokyoJapan

Personalised recommendations