, Volume 97, Issue 6, pp 481–487 | Cite as

Polymorphism in the location of the 18S and 28S rRNA genes on the chromosomes of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor

  • J. E. Wiley
  • M. L. Little
  • M. A. Romano
  • D. A. Blount
  • G. R. Cline


The chromosomes of the diploid Hyla chrysoscelis and its tetraploid sibling species H. versicolor were studied with AgNO3 staining and in situ hybridization to determine the chromosome location of the 18S and 28S ribosomal RNA (rRNA) genes. A total of 236 Hyla chrysoscelis from 34 localities in 15 U.S. states and 100 H. versicolor from 15 localities in 12 states were examined. The rRNA gene sites were extremely variable in H. chrysoscelis, and also variable, but to a lesser extent, in H. versicolor. The most common rRNA gene site in both H. chrysoscelis and H. versicolor was on the short arm of chromosome 6. All of the rRNA gene locations seen in H. versicolor were also seen in H. chrysoscelis, supporting the hypothesis that the tetraploid H. versicolor arose from H. chrysoscelis. Although polymorphic rRNA gene sites in H. versicolor may reflect the positions of the rRNA genes in H. chrysoscelis ancestors, the origin of the extreme variability of such sites in H. chrysoscelis seems more obscure. Possible explanations include inversions, translocations, mobile genetic elements or a combination of some or all of these.


Developmental Biology AgNO3 Gene Location Chromosome Location Genetic Element 
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  1. Bogart JP, Wasserman AO (1972) Diploid-polyploid cryptic species pairs: a possible clue to evolution by polyploidisation in anuran amphibians. Cytogenetics 11:7–24Google Scholar
  2. Erickson JM, Rushford CL, Dorney DJ, Wilson GN, Schmickel RD (1981) Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. Gene 16:1–9Google Scholar
  3. Georgiev GP (1984) Mobile genetic elements in animal cells and their biological significance. Eur J Biochem 145:203–220Google Scholar
  4. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014Google Scholar
  5. Langer ER, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labelled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637Google Scholar
  6. Langer-Safer PR, Levine M, Ward DC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci USA 79:4381–4385Google Scholar
  7. Lohmann K, Kraus K (1985) Are there insertions in the ribosomal DNA of vertebrates? Nucleic Acids Res 13:5145–5155Google Scholar
  8. Long KO, Dawid IB (1980) Repeated genes in eukaryotes. Annu Rev Biochem 49:727–764Google Scholar
  9. Maxson L, Pepper E, Maxson RD (1977) Immunological resolution of a diploid-tetraploid species complex of tree frogs. Science 197:1012–1013Google Scholar
  10. Nardi I, Barsacchi-Pilone G, Batistoni R, Andronico F (1977) Chromosome location of the ribosomal RNA genes in Triturus vulgaris meridionalis (Amphibia, Urodele). Chromosoma 64:67–84Google Scholar
  11. Ralin DB, Selander RK (1979) Evolutionary genetics of diploid-tetraploid species of treefrogs of the genus Hyla. Evolution 33:595–608Google Scholar
  12. Ralin DB, Romano MA, Kirkpatrick CW (1983) The tetraploid treefrog Hyla versicolor: evidence for a single origin from the diploid Hyla chrysoscelis. Herpetologica 39:212–225Google Scholar
  13. Syvanen M (1984) The evolutionary implications of mobile genetic elements. Annu Rev Genet 18:271–273Google Scholar
  14. Wiley JE (1982) Chromosome banding patterns of treefrogs (Hylidae) of the eastern United States. Herpetologica 38:507–520Google Scholar
  15. Wiley JE (1983) Chromosome polymorphism in Hyla chrysoscelis. Copeia 1983:273–275Google Scholar
  16. Wiley JE, Meisner LF (1984) Synergistic effect of TPA and T-cell mitogens in nonmammalian vertebrates. In Vitro 20:932–936Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. E. Wiley
    • 1
  • M. L. Little
    • 2
  • M. A. Romano
    • 3
  • D. A. Blount
    • 1
  • G. R. Cline
    • 4
  1. 1.Department of Pediatrics, Division of Medical Genetics, Brody 3N51East Carolina University School of MedicineGreenvilleUSA
  2. 2.Department of Biological SciencesMarshall UniversityHuntingtonUSA
  3. 3.Department of Biological SciencesWestern Illinois UniversityMacombUSA
  4. 4.Department of ZoologyOklahoma State UniversityStillwaterUSA

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