, Volume 97, Issue 6, pp 421–428 | Cite as

A retroposon-like short repetitive DNA element in the genome of the human blood fluke, Schistosoma mansoni

  • Loretta D. Spotila
  • Hirohisa Hirai
  • David M. Rekosh
  • Philip T. LoVerde


The genome of Schistosoma mansoni, a human blood fluke, contains a family of short repetitive DNA elements which we have named the SMα family. In this paper we report the sequences of two SMα family members which are derived from tandem arrangements and four family members which are dispersed copies. The two tandemly repeated copies are 331 and 335 bp, while the four dispersed copies range in size from 107 to 322 bp. Three dispersed copies are flanked by direct repeats and have AT-rich 3′ ends. The tandem copies and one of the dispersed copies have regions of homology to RNA polymerase III promoters and arginine tRNA genes. In addition the repeated element is rearranged in two of the dispersed copies when compared with the other dispersed and two tandem copies. Localization studies show that SMα elements are distributed in the sex and autosomal chromosomes. These observations suggest that members of this family may have been dispersed throughout the genome via RNA intermediates.


Family Member Arginine Developmental Biology Localization Study tRNA Gene 
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  1. Adams DS, Eickbush TH, Herrera RJ, Lizardi PM (1986) A highly reiterated family of transcribed oligo(A)-termmated, interspersed DNA elements in the genome of Bombyx mori. J Mol Biol 197:465–478Google Scholar
  2. Bobek LA, Rekosh DM, LoVerde PT (1987) Isolation and analysis of adult-female-specific genes from three species of human schistosome parasites. In: MacInnis A (ed) Molecular paradigms for eradicating helminthic parasites. Alan R. Liss, New York, pp 149–158Google Scholar
  3. Daniels GR, Deininger PL (1985) Integration site preferences of the Alu family and similar repetitive DNA sequences. Nucleic Acids Res 13:8939–8954Google Scholar
  4. Devereux P, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–391Google Scholar
  5. Dover GA (1986) Molecular drive in multigene families: how biological novelties arise, spread and are assimilated. Trends Genet 2:159–165Google Scholar
  6. Durnam DM, Menninger JC, Chandler SH, Smith PP, McDougall JK (1988) A fragile site in the human U2 small nuclear RNA gene cluster is revealed by adenovirus Type 12 infection. Mol Cell Biol 8:1863–1867Google Scholar
  7. Endoh H, Okada N (1986) Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures. Proc Natl Acad Sci USA 83:251–255Google Scholar
  8. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13Google Scholar
  9. Harper ME, Saunders GF (1981) Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization. Chromosoma 83:431–439Google Scholar
  10. Hirai H, Sakaguchi Y, Habe S, Imai HT (1985) C-banding analysis of six species of lung flukes, Paragonimus spp. (Trematoda: Platyhelminthes), from Japan and Korea. Z Parasitenkd 71:617–629Google Scholar
  11. Jagadeeswaran P, Forget BG, Weissman SM (1981) Short interspersed repetitive DNA elements in eucaryotes: transposable DNA elements generated by reverse transcrition of RNA pol III transcripts? Cell 26:141–142Google Scholar
  12. Jelinek WR, Schmid CR (1982) Repetitive sequences in eukaryotic DNA and their expression. Annu Rev Biochem 51:813–844Google Scholar
  13. Leary JJ, Brigati DJ, Ward DC (1983) Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bioblots. Proc Natl Acad Sci USA 80:4045–4049Google Scholar
  14. Matsumoto K-I, Murakami K, Okada N (1986) Gene for lysine tRNA may be a progenitor of the highly repetitive and transcribable sequences present in the salmon genome. Proc Natl Acad Sci USA 83:3156–3160Google Scholar
  15. Meyerhof W, Wittig B, Tappeser B, Knochel W (1987) Transcription termination and processing of transcripts from tRNA-related Xenopus satellite DNA sequences. Eur J Biochem 164:287–293Google Scholar
  16. Narayanswami S, Hamkalo BA (1986) Electron microscopic in situ hybridization using biotinylated probes. Focus 8:3–6Google Scholar
  17. Nisson PE, Hickey RJ, Boshar MF, Crain WR (1988) Identification of a repeated sequence in the genome of the sea urchin which is transcribed by RNA polymerase III and contains the features of a retroposon. Nucleic Acids Res 16:1431–1452Google Scholar
  18. Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938Google Scholar
  19. Rogers JH (1985) Origin and evolution of retroposons. Int Rev Cytol 93:187–279Google Scholar
  20. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  21. Short R (1983) Sex and the single schistosome. J Parasitol 69:4–22Google Scholar
  22. Short R, Grossman AI (1981) Conventional giemsa and C-banded karyotypes of Schistosoma mansoni and S. rodhaini. J Parasitol 67:661–671Google Scholar
  23. Simpson AGJ, Sher A, McCutchan TF (1982) The genome of Schistosoma mansoni: isolation of DNA, its size, bases, and repetitive sequences. Mol Biochem Parasitol 6:125–137Google Scholar
  24. Singer MF (1982) SINEs and LINEs: highly repeated short and long interspersed sequences in mammalian genomes. Cell 28:433–444Google Scholar
  25. Spotila LD, Rekosh DM, Boucher JM, LoVerde PT (1987a) A cloned DNA probe identifies the sex of Schistosoma mansoni cercariae. Mol Biochem Parasitol 26:17–20Google Scholar
  26. Spotila LD, LoVerde PT, Rekosh DM (1987b) Analysis of two repeated DNA sequences of Schistosoma mansoni. In: MacInnis A (ed) Molecular paradigms for eradicating helminthic parasites. Alan R. Liss, New York, pp 159–168Google Scholar
  27. Sprinzl M, Moll J, Meissner F, Hartmann T (1985) A collection of tRNA, 5S, 5.8S rRNA small RNA and restriction enzyme recognition site sequences. Nucleic Acids Res 13:1–50Google Scholar
  28. Ullu E (1982) The human Alu family of repeated DNA sequences. Immunology 11:71–75Google Scholar
  29. Weiner AM, Deininger PL, Efstratiadis A (1986) Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem 55:631–661Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Loretta D. Spotila
    • 1
    • 2
  • Hirohisa Hirai
    • 1
  • David M. Rekosh
    • 1
    • 2
  • Philip T. LoVerde
    • 1
  1. 1.Department of MicrobiologyState University of New York at BuffaloBuffaloUSA
  2. 2.Department of BiochemistryState University of New York at BuffaloBuffaloUSA

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