, Volume 73, Issue 1, pp 117–129 | Cite as

Folded chromosomes of mating-factor arrested yeast cells: comparison with G0 arrest

  • R. Piñon
  • D. Pratt


Folded chromosomes of haploid a or diploid aa cells arrested by α-factor are characterized by a heterogeneous sedimentation distribution, distinguishable from the corresponding g0and g1 structures. The extent of heterogeneity appears to depend in part on the extent of the characteristic schmoe morphology. Under conditions where a and α mating-type cells are mixed together without conjugation, folded chromosomes characteristic of α-factor arrested a and aa cells appear in both cell types in a synchronous manner. Attempts to order G0 and α-factor arrest suggest that these two arrest stages represent two different branch pathways out of the cell cycle. These two branch pathways may have a common branch point, or they may be characterized by two different branch points.


Cell Cycle Yeast Cell Developmental Biology Branch Point Branch Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Bücking-Throm, E., Duntze, W., Hartwell, L.H., Manney, T.R.: Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor. Exp. Cell Res. 76, 99–110 (1973)Google Scholar
  2. Byers, B., Goetsch, L.: Duplication of spindle plaques and integration of the yeast cell cycle. Cold Spr. Harb. Symp. quant. Biol. 38, 123–131 (1974)Google Scholar
  3. Hartwell, L.H.: Synchronization of haploid yeast cell cycle, a prelude to conjugation. Exp. Cell Res. 76, 111–117 (1973)Google Scholar
  4. Hartwell, L.H.: Cell division from a genetic perspektive. J. Cell Biol. 77, 627–637 (1978)Google Scholar
  5. Hereford, L.M., Hartwell, L.H.: Sequential gene function in the initiation of S. cerevisiae DNA synthesis. J. molec. Biol. 84, 445–461 (1974)Google Scholar
  6. Jarvik, J., Botstein, D.: A genetic method for determining the order of events in a biological pathway. Proc. nat. Acad. Sci. (Wash.) 70, 2046–2050 (1973)Google Scholar
  7. Johnston, G.C., Pringle, J.R., Hartwell, L.H.: Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp. Cell Res. 105, 74–98 (1976)Google Scholar
  8. Piñon, R.: Folded chromosomes in non-cycling yeast cells: evidence for a characteristic g0 form. Chromosoma (Berl.) 67, 263–274 (1978)Google Scholar
  9. Piñon, R., Salts, Y.: Isolation of folded chromosomes from the yeast Saccharomyces cerevisiae. Proc. nat. Acad. Sci. (Wash.) 74, 2850–2854 (1977)Google Scholar
  10. Piñon, R., Salts, Y., Simchen, G.: Nuclear and mitochondrial DNA synthesis during yeast sporulation. Exp. Cell Res. 83, 231–238 (1974)Google Scholar
  11. Simchen, G., Piñon, R., Salts, Y.: Sporulation in Sacchromyces cerevisiae: Premeiotic DNA synthesis, readiness, and commitment. Exp. Cell Res. 79, 207–218 (1972)Google Scholar
  12. Wilkinson, L.E., Pringle, J.R.: Transient G1 arrest of S. cerevisiae cells of mating type a by α factor produced by cells of mating type a. Exp. Cell Res. 89, 175–187 (1974)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. Piñon
    • 1
  • D. Pratt
    • 1
  1. 1.Department of BiologyUniversity of CaliforniaSan Diego, La JollaUSA

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