Current Microbiology

, Volume 31, Issue 1, pp 34–38 | Cite as

Characterization of Methanosarcina mazeii TMA isolated from a paddy field soil

  • Susumu Asakawa
  • Masayo Akagawa-Matsushita
  • Hiroyuki Morii
  • Yosuke Koga
  • Koichi Hayano

Abstract

We isolated a methanogenic strain, designated as strain TMA (=DSM 9195), from an enrichment culture inoculated with a Japanese paddy field soil. Strain TMA was Gram positive and strictly anaerobic. Cell shape was pseudosarcina-like, and cells were nonmotile. The strain was able to use methylamines, methanol, H2−CO2, and acetate as substrates for methanogenesis, but did not utilize formate. The optimum temperature and optimum pH were 30–37°C and 6.5–7.5 respectively. The G+C content of the DNA was 42.1 mol %. Strain TMA had DNA-DNA hybridization values of more than 80% with Methanosarcina mazeii S-6T (T = type strain). On the basis of phenotypic and genotypic characteristics, we identified strain TMA as M. mazeii. This is the first methylotrophic methanogen isolated from a paddy field soil and identified to the species level.

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Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Susumu Asakawa
    • 1
  • Masayo Akagawa-Matsushita
    • 2
  • Hiroyuki Morii
    • 2
  • Yosuke Koga
    • 2
  • Koichi Hayano
    • 1
  1. 1.Laboratory of Soil MicrobiologyKyushu National Agricultural Experiment StationNishigoshi, KumamotoJapan
  2. 2.Department of ChemistryUniversity of Occupational and Environmental Health, JapanKitakyushuJapan
  3. 3.Laboratory of Soil Organic ChemistryNational Institute of Agro-Environmental SciencesTsukubaJapan

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