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Acta Neuropathologica

, Volume 89, Issue 1, pp 2–7 | Cite as

In vitro ischemia-induced intracellular Ca2+ elevation in cerebellar slices: a comparative study with the values found in hippocampal slices

  • Akira Mitani
  • Hisato Yanase
  • Shigeru Namba
  • Masachika Shudo
  • Kiyoshi Kataoka
Regular Paper

Abstract

Changes in levels of intracellular calcium ion ([Ca2+]i) induced by in vitro ischemic conditions in gerbil cerebellar and hippocampal slices were investigated using a calcium imaging system and electron microscopy. When the cerebellar slice was perfused with a glucose-free physiological medium equilibrated with a 95% N2/5% CO2 gas mixture (in vitro ischemic medium), a large [Ca2+]i elevation was region-specifically induced in the molecular laver of the cerebellar cortex (a dendritic field of Purkinje cells). When the hippocampal slice was perfused with in vitro ischemic medium, a large [Ca2+]i elevation was region-specifically induced in CA1 field of the hippocampal slices. Electron microscopic examinations showed that the large [Ca2+]i elevations occurred in Purkinje cells and CA1 pyramidal neurons. To isolate Ca2+ release from intracellular Ca2+ store sites, the slices were perfused with Ca2+-free in vitro ischemic medium. the increases in [Ca2+]i in both cerebellar and hippocampal slices were significantly lower than those observed in the slices perfused with the Ca2+-containing in vitro ischemic medium. However, the suppression of the [Ca2+]i-elevation in the molecular layer of the cerebellar slices was smaller than that in the CA1 field of the hippocampal slices. These results reinforce the hypothesis that calcium plays a pivotal role in the development of ischemia-induced neuronal death, and suggest that Ca2+ release from intracellular Ca2+ store sites may play an important role in the ischemia-induced [Ca2+]i elevation in Purkinje cells.

Key words

Calcium Ischemia Cerebellum Purkinje cell Microfluorometry 

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Akira Mitani
    • 1
  • Hisato Yanase
    • 1
  • Shigeru Namba
    • 1
  • Masachika Shudo
    • 2
  • Kiyoshi Kataoka
    • 1
  1. 1.Department of PhysiologyEhime University School of MedicineEhimeJapan
  2. 2.The central Research LaboratoriesEhime University School of MedicineEhimeJapan

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